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CRISPR/Cas9-mediated editing of the TM6 MADS-box gene in the octoploid strawberry (Fragaria x ananassa)
dc.contributor.author | Martín-Pizarro, Carmen | |
dc.contributor.author | Posé-Padilla, David | |
dc.date.accessioned | 2019-05-10T07:40:55Z | |
dc.date.available | 2019-05-10T07:40:55Z | |
dc.date.created | 2019 | |
dc.date.issued | 2019-05-10 | |
dc.identifier.uri | https://hdl.handle.net/10630/17634 | |
dc.description | El contenido del poster presentado está desarrollado en: doi:10.1093/jxb/ery400 y Publicado por Oxford University press | en_US |
dc.description.abstract | The B-class of MADS-box transcription factors has been studied in many plant species, but remain functionally uncharacterized in the Rosaceae family. APETALA3 (AP3), a member of this class, controls the identity of petals and stamens in Arabidopsis thaliana. In this work, we identified two members of the AP3 lineage in the cultivated strawberry (Fragaria × ananassa): FaAP3 and FaTM6. Interestingly, FaTM6, and not FaAP3, shows an expression pattern equivalent to that of AP3 in Arabidopsis. Genome editing using Cluster Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is becoming a robust tool for targeted and stable mutagenesis of DNA. However, whether it can be efficiently used in an octoploid species such as F. × ananassa is not known. In our study, we report the application of the CRISPR/Cas9 in F. × ananassa to characterize the function of FaTM6 in flower development. An exhaustive analysis by high-throughput sequencing of the FaTM6 locus spanning the target sites showed a high efficiency genome editing already in the T0 generation. The phenotypic characterization of the mutant lines indicates that FaTM6 plays a key role in petal and especially in anther development in strawberry. Our results validate the CRISPR/Cas9 strategy for gene functional analysis in an octoploid species such as F. × ananassa, and offer new opportunities for engineering strawberry to improve traits of interest in breeding programs. | en_US |
dc.description.sponsorship | Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Ingeniería genética vegetal | en_US |
dc.subject.other | Strawberry | en_US |
dc.subject.other | Fragaria x ananassa | en_US |
dc.subject.other | Flower | en_US |
dc.subject.other | TM6 | en_US |
dc.subject.other | CRISPR/Cas9 | en_US |
dc.title | CRISPR/Cas9-mediated editing of the TM6 MADS-box gene in the octoploid strawberry (Fragaria x ananassa) | en_US |
dc.type | info:eu-repo/semantics/conferenceObject | en_US |
dc.centro | Facultad de Ciencias | en_US |
dc.relation.eventtitle | At the Forefront of Plant Research 2019 | en_US |
dc.relation.eventplace | Barcelona | en_US |
dc.relation.eventdate | 6-8 Mayo 2019 | en_US |
dc.rights.cc | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |