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dc.contributor.authorMartín-Pizarro, Carmen
dc.contributor.authorPosé, David
dc.date.accessioned2019-05-10T07:40:55Z
dc.date.available2019-05-10T07:40:55Z
dc.date.created2019
dc.date.issued2019-05-10
dc.identifier.urihttps://hdl.handle.net/10630/17634
dc.descriptionEl contenido del poster presentado está desarrollado en: doi:10.1093/jxb/ery400 y Publicado por Oxford University pressen_US
dc.description.abstractThe B-class of MADS-box transcription factors has been studied in many plant species, but remain functionally uncharacterized in the Rosaceae family. APETALA3 (AP3), a member of this class, controls the identity of petals and stamens in Arabidopsis thaliana. In this work, we identified two members of the AP3 lineage in the cultivated strawberry (Fragaria × ananassa): FaAP3 and FaTM6. Interestingly, FaTM6, and not FaAP3, shows an expression pattern equivalent to that of AP3 in Arabidopsis. Genome editing using Cluster Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is becoming a robust tool for targeted and stable mutagenesis of DNA. However, whether it can be efficiently used in an octoploid species such as F. × ananassa is not known. In our study, we report the application of the CRISPR/Cas9 in F. × ananassa to characterize the function of FaTM6 in flower development. An exhaustive analysis by high-throughput sequencing of the FaTM6 locus spanning the target sites showed a high efficiency genome editing already in the T0 generation. The phenotypic characterization of the mutant lines indicates that FaTM6 plays a key role in petal and especially in anther development in strawberry. Our results validate the CRISPR/Cas9 strategy for gene functional analysis in an octoploid species such as F. × ananassa, and offer new opportunities for engineering strawberry to improve traits of interest in breeding programs.en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectIngeniería genética vegetalen_US
dc.subject.otherStrawberryen_US
dc.subject.otherFragaria x ananassaen_US
dc.subject.otherFloweren_US
dc.subject.otherTM6en_US
dc.subject.otherCRISPR/Cas9en_US
dc.titleCRISPR/Cas9-mediated editing of the TM6 MADS-box gene in the octoploid strawberry (Fragaria x ananassa)en_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleAt the Forefront of Plant Research 2019en_US
dc.relation.eventplaceBarcelonaen_US
dc.relation.eventdate6-8 Mayo 2019en_US
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*


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