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dc.contributor.authorValderrama-Martín, José Miguel
dc.contributor.authorOrtigosa, Francisco
dc.contributor.authorÁvila Sáez, Concepción
dc.contributor.authorCanovas-Ramos, Francisco Miguel 
dc.contributor.authorCañas, Rafael Antonio
dc.date.accessioned2019-07-08T12:01:45Z
dc.date.available2019-07-08T12:01:45Z
dc.date.created2019
dc.date.issued2019-07-08
dc.identifier.urihttps://hdl.handle.net/10630/17986
dc.description.abstractNitrogen is an essential element for life and the main limiting nutrient for plant growth and development1. The main forms of inorganic nitrogen in soils are nitrate and ammonium, which relative abundances depend on environmental conditions such as temperature. In agricultural soils the most abundant nitrogen form is nitrate because the use of chemical fertilizers however in natural ecosystems nitrogen soil composition can be more complex. Conifers are tree gymnosperms with a wide distribution although their large forests dominate the boreal ecosystems where nitrification is limited and ammonium is the main nitrogen soil source2. In this context, conifers have an appreciable tolerance to ammonium. Maritime pine (Pinus pinaster Aiton) is a conifer from the western Mediterranean region of high economic and ecological interest in Spain, France and Portugal. This pine is also a research model tree with different genomic resources such as a reference transcriptome and a gene expression atlas3. Taking advantage of these resources the members of the NPF and NRT transporter families involved in nitrate uptake and transport have been identified and analyzed in maritime pine4. Among the transporter families, the NRT3 one is expanded and composed by six members. The capacity of maritime pine to use nitrate or ammonium has been analyzed in seedlings. The development and growth responses to nitrate nutrition are comparable to ammonium supply. At molecular level, there are strong gene expressions for genes involved in nitrate uptake and assimilation such as Nitrate Reductase, Nitrite Reductase, Glutamine Synthetase 1a, three NRT3 genes and different NPF family members in the different organs. Since the NPF proteins can transport different metabolites, peptides and hormones, the NPF transporters involved in nitrate transport are being identified.en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. This project was supported by the grant MicroNUpE, BIO2015-73512-JIN; MINECO/AEI/FEDER, UE. JMVM was supported by a grant from the Spanish Ministerio de Educación y Formación Profesional (FPU17/03517) and FO by a grant from the Universidad de Málaga (Programa Operativo de Empleo Juvenil vía SNJG, UMAJI11, FEDER, FSE, Junta de Andalucía).en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNitrógeno -- Metabolismoen_US
dc.subjectConíferasen_US
dc.subjectNitratoen_US
dc.subjectFisiología vegetalen_US
dc.subject.otherNitrogen metabolismen_US
dc.subject.otherConiferen_US
dc.subject.otherNitrateen_US
dc.subject.otherTransporteren_US
dc.titleAnalysis of NPF and NRT transporter families regarding the nitrate nutrition in maritime pine (Pinus pinaster)en_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleXXIII Meeting of the Spanish Society of Plant Physiology / XVI Spanish Portuguese Congress of Plant Physiologyen_US
dc.relation.eventplacePamplonaen_US
dc.relation.eventdate26 junio 2019en_US


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