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dc.contributor.authorPose, S.
dc.contributor.authorRic-Varas, P.
dc.contributor.authorSchückel, J.
dc.contributor.authorMolina-Hidalgo, F.J.
dc.contributor.authorBlanco-Portales, R.
dc.contributor.authorMuñoz-Blanco, J.
dc.contributor.authorKnox, P.
dc.contributor.authorMatas, A.J.
dc.contributor.authorQuesada, M.A.
dc.contributor.authorMercado, J.A.
dc.date.accessioned2019-07-23T09:47:09Z
dc.date.available2019-07-23T09:47:09Z
dc.date.created2019
dc.date.issued2019-07-23
dc.identifier.urihttps://hdl.handle.net/10630/18121
dc.description.abstractStrawberry fruits greatly reduce their quality due to softening during ripening with economically important losses. Texture changes of fleshy fruits during ripening are mainly due to middle lamellae dissolution, cell-to-cell adhesion losses and wall weakening of parenchyma cells by the coordinated action of several cell wall enzymes. Pectin degradation has been proven a key factor in strawberry softening by functional analysis of several pectinase genes (polygalacturonase, pectate lyase and -galactosidase). The complexity and highly dynamic nature of pectins remains a challenge to fully elucidate structure-function relationships of pectins. In this work, we present the functional analysis of two independent strawberry transgenic lines with more than 95% silencing of a rhamnogalacturonate lyase gene (FaRGLyase1). Firmness of ripe fruit was significantly higher in both transgenic lines than in the control. Cell walls from these fruits were extracted and analyzed by glycan microarray profiling. This high‐throughput technique allows a wide screening of cell-wall glycan occurrence based on the detection of specific cell wall oligosaccharide epitopes by monoclonal antibodies and reveals profiles which can be used as potential fingerprints specific for a singular organ and/or developmental stage. Our microarray results showed that the silencing of FaRGLyase1 reduced degradation of several rhamnogalacturonan-I related epitopes, as expected. Additionally, comparison of transgenic cell walls from ripe fruits with those extracted from control fruits at different developmental stages (green, white and red) by hierarchical clustering, demonstrated a higher similarity of transgenic fruit cell walls with the control cell walls from fruits at the white stage. Glycan microarray profiles revealed less degraded fruit cell walls as result of FaRGLyase1 down-regulation which could contribute to the increased firmness of transgenic fruitsen_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectParedes celulares vegetalesen_US
dc.subjectFresasen_US
dc.subjectPectinaen_US
dc.subject.otherStrawberryen_US
dc.subject.otherCell wallen_US
dc.subject.otherFruit ripeningen_US
dc.subject.otherFruit softeningen_US
dc.subject.otherPectinen_US
dc.titleCell wall disassembly is delayed by rhamnogalacturonate lyase gene silencing: potential role in fruit firmnessen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleXV Cell Wall Meetingen_US
dc.relation.eventplaceCambridge, Reino Unidoen_US
dc.relation.eventdateJulio, 2019en_US


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