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dc.contributor.authorLuna, Ana P.
dc.contributor.authorRodríguez Negrete, Edgar A.
dc.contributor.authorMorilla, Gabriel
dc.contributor.authorCastillo-Garriga, Araceli 
dc.contributor.authorRodriguez-Bejarano, Eduardo 
dc.date.accessioned2019-07-25T07:19:56Z
dc.date.available2019-07-25T07:19:56Z
dc.date.created2019
dc.date.issued2019-07-25
dc.identifier.urihttps://hdl.handle.net/10630/18133
dc.description.abstractGeminiviruses constitute a group of plant viruses that infect vegetable crops all over the world. Among the Geminiviridae family, the genera Mastrevirus, Begomovirus and Curtovirus are the most abundant. Suppression of gene silencing is a key mechanism for viral infection in plants. In begomovirus, V2 is a strong posttranscriptional gene silencing suppressor. We recently showed that V2 from curtovirus Beet curly top virus (BCTV) is a PTGS suppressor by impairing the RDR6/SGS3 pathway, as V2 from begomovirus. In order to identify the domains involved in the suppression activity and viral pathogenicity, we performed an alignment of several begomovirus and curtovirus V2 proteins. A protein kinase C (PKC) phosphorylation motif essential for suppression activity in begomovirus (P1) was found in all analysed sequences. We also found similar hydrophobic profiles, with two hydrophobic domains (H1 and H2) followed by a long hydrophilic domain. Then we generated BCTV V2 mutant proteins and performed transient assays in Nicotiana benthamiana plants to test their suppression activity. We also expressed them from a Potato virus X-derived vector to check the symptoms produced. Additionally, their subcellular localization was determined. Finally, we produced BCTV viruses mutated in the different domains and N. benthamiana plants were infected, analysing virus levels and symptoms produced. The results showed that P1, H1 and H2 are involved in the suppression activity and viral pathogenicity.en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Techen_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGenética - Congresosen_US
dc.subject.otherPTGSen_US
dc.subject.otherGeminivirusen_US
dc.titleStudy of the functional domains of the PTGS suppressor V2 from geminivirus Beet curly top virus (BCTV)en_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitleIS-MPMI XVIII Congressen_US
dc.relation.eventplaceGlasgow, Escociaen_US
dc.relation.eventdateJulio 2019en_US


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