JavaScript is disabled for your browser. Some features of this site may not work without it.

    Listar

    Todo RIUMAComunidades & ColeccionesPor fecha de publicaciónAutoresTítulosMateriasTipo de publicaciónCentrosDepartamentos/InstitutosEditoresEsta colecciónPor fecha de publicaciónAutoresTítulosMateriasTipo de publicaciónCentrosDepartamentos/InstitutosEditores

    Mi cuenta

    AccederRegistro

    Estadísticas

    Ver Estadísticas de uso

    DE INTERÉS

    Datos de investigaciónReglamento de ciencia abierta de la UMAPolítica de RIUMAPolitica de datos de investigación en RIUMAOpen Policy Finder (antes Sherpa-Romeo)Dulcinea
    Preguntas frecuentesManual de usoContacto/Sugerencias
    Ver ítem 
    •   RIUMA Principal
    • Investigación
    • Ponencias, Comunicaciones a congresos y Pósteres
    • Ver ítem
    •   RIUMA Principal
    • Investigación
    • Ponencias, Comunicaciones a congresos y Pósteres
    • Ver ítem

    The limit of sulphide adaptation in the sulphide-sensitive cyanobacterium "Microcystis aeruginosa"

    • Autor
      Martín-Clemente, Elena; Melero-Jiménez, Ignacio José; Bañares-España, ElenaAutoridad Universidad de Málaga; Flores-Moya, AntonioAutoridad Universidad de Málaga; García-Sánchez, María JesúsAutoridad Universidad de Málaga
    • Fecha
      2019
    • Editorial/Editor
      Universidad de Málaga/Sociedad Española de Microbiología
    • Palabras clave
      Cianobacterias; Sulfuros; Microbiología -- Congresos
    • Resumen
      Although sulphide has played an important role in the evolution of photosynthesis, it produces a lethal effect on most photosynthetic organisms due to its redox activity on certain enzymes, inhibiting oxygenic photosynthetic and respiratory electron transport. However, cyanobacteria vary in sulphide tolerance, showing different degrees of sulphide resistant oxygenic photosynthesis and even some taxa have the capacity to perform anoxygenic sulphide-dependent photosynthesis. Microcystis aeruginosa is a sulphide-sensitive species. However, it is known that genetic adaptation of aquatic photosynthetic microorganisms to selective agents can be rapidly achieved, even at lethal levels, as the consequence of single mutations. The aim of this work was to determine the maximum sulphide concentration to which this sulphide-sensitive species is able to adapt in order to shed light on the process of sulphide adaptation in cyanobacteria. We used three M. aeruginosa strains, Ma1Vc, Ma5Vc and MaAVc, isolated from a non-sulphureous environment, and whose lethal doses were 0.10, 0.16 and 0.20 mM sulphide, respectively. To study the adaptation of these strains to sulphide a modified ratchet experiment was carried out [...]. The M. aeruginosa strains reached different limits of sulphide adaptation. Ma5Vc and Ma1Vc strains adapted up to 0.40 mM sulphide, i.e. 2,5-fold and 4-fold their initial lethal doses, respectively. However, the MaAVc strain, which showed an initial higher sulphide tolerance, adapted up to only 0.27 mM. Sulphide-lethal dose and photosynthetic performance of the resistant strains obtained in the ratchet experiment were characterized. The lethal dose of the M. aeruginosa Ma1Vc and Ma5Vc mutant strains was 0.40 and 0.72 mM sulphide, respectively. These resistant strains showed lower growth and photosynthetic rates than wild-type ones in the absence of sulphide, which indicates the physiological cost of the mutation conferring sulphide resistance.
    • URI
      https://hdl.handle.net/10630/18136
    • Compartir
      RefworksMendeley
    Mostrar el registro completo del ítem
    Ficheros
    Martin_Clemente_et_al_2019.pdf (62.30Kb)
    Colecciones
    • Ponencias, Comunicaciones a congresos y Pósteres

    Estadísticas

    Buscar en Dimension
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
     

     

    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA