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dc.contributor.authorLeiva-Rebollo, Rocío
dc.contributor.authorLabella Vera, Alejandro Manuel
dc.contributor.authorCastro-López, María Dolores 
dc.contributor.authorBorrego-García, Juan José 
dc.date.accessioned2019-07-29T08:49:05Z
dc.date.available2019-07-29T08:49:05Z
dc.date.created2019
dc.date.issued2019-07-29
dc.identifier.urihttps://hdl.handle.net/10630/18168
dc.description.abstractLymphocystis disease (LCD) is caused by the lymphocystis disease virus (LCDV) (family Iridoviridae), affecting more than 150 fish species from both marine and freshwater environments. A few studies have been focused on the immune defensive mechanisms of fish against LCDV, but only one was conducted during a natural LCD outbreak in gilthead seabream, which is one of the most important cultured fish species in the Mediterranean and the European Atlantic coasts. The aim of this study was the analysis of 23 genes related to the immune response in gilthead seabream specimens after experimental infection with LCDV-Sa using real-time PCR (qRT-PCR) in samples of head kidney and intestine at 1, 3, and 8 dpi. To study the progression of LCDV-Sa infection in gilthead seabreams, the number of viral DNA copies and the expression of mcp were determined in samples of caudal fin, head kidney and intestine. LCDV-Sa was detected by qPCR in all the samples from inoculated fish analysed, whereas no amplification was obtained in samples from the control group. Regarding the gene expression following LCDV-Sa infection, a total of 22 of the 23 genes studied were differentially expressed in head kidney or intestine samples at some time points analysed. Different gene expression profiles were obtained between the organs studied, detecting 18 differentially expressed genes (DEGs) in head kidney samples, four of them exclusively up- or down-regulated (nccrp1, il10, mhcII, and tnfα genes), and 5 genes with a significant change in the expression tendency from 1 to 8 dpi (irf3, isg15, il10, ck10, and c3). In the intestine, 18 DEGs were also detected (14 shared with head kidney), being mx1, casp1, ck3 and tlr9 genes exclusively detected in these samples, and mx1, mx3, irf9 and ighm differentially regulated over time. The results obtained allow us to understand which genes are essential for host-pathogen interactions and could be used as molecular markers for vaccine efficacy evaluation.en_US
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech Proyecto de Excelencia Junta de Andalucía Ref. P12-RNM-2261en_US
dc.language.isoengen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleImmune response of gilthead seabream (Sparus aurata) after experimental infection with lymphocystis disease virus (LCDV-Sa)en_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.centroFacultad de Cienciasen_US
dc.relation.eventtitle3rd International Conference of Fish & Shellfish Immunologyen_US
dc.relation.eventplaceLas Palmas de Gran Canaria (Spain)en_US
dc.relation.eventdateJunio 2019en_US


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