Nitrogen, in natural environments, is the main limiting nutrient for plant growth and development1. This
essential element can be presented in different forms in the soils being the two main forms nitrate and
ammonium, although the relative abundance of these compounds depends on environmental conditions.
In plants, nitrate uptake and transport involve the function of specific transporters, mainly of the NRT and
NPF families. Genes encoding these transporters have been well studied in crop plants since in
agricultural soils nitrate has become the predominant nitrogen compound as a result of the intensive use
of fertilizers2. However, in soils of the large conifer forests dominating the boreal ecosystems,
ammonium is the main source of inorganic nitrogen due to the limited nitrification process3.
Consequently, conifers have been told to have a preference for ammonium over nitrate and only limited
information is available about nitrate transporters in these gymnosperms.
Maritime pine (Pinus pinaster Aiton) is a conifer tree with a wide distribution in the western
Mediterranean region and with a great morphological and physiological plasticity. This pine also has a
good resistance to abiotic stress4. In maritime pine, the genomic resources have been improved in the
last few years5 allowing the identification and molecular analysis of members of the NRT and NPF gene
families6.
Growth and development responses have been compared on pine seedlings cultured under different
nitrate and ammonium supplies. Gene expression have been analyzed and the results show a strong
expression of different genes related with the uptake, transport and assimilation of nitrate in plants such
as nitrate and nitrite reductases, glutamine synthetase and some genes from the NRT and NPF families,
suggesting they are involved in nitrate acquisition from soil.