Mostrar el registro sencillo del ítem

dc.contributor.authorSánchez-Gómez, Carlos
dc.contributor.authorMeco, Victoriano
dc.contributor.authorUrrutia, María
dc.contributor.authorCastillo, Araceli G.
dc.contributor.authorPérez-Sancho, Jessica
dc.contributor.authorBayer, Emmanuelle M.
dc.contributor.authorFranco-Zorrila, José M.
dc.contributor.authorMartín-Pizarro, Carmen
dc.contributor.authorPosé, David
dc.date.accessioned2021-01-22T10:29:18Z
dc.date.available2021-01-22T10:29:18Z
dc.date.created2020-12-09
dc.date.issued2021-01-22
dc.identifier.urihttps://hdl.handle.net/10630/20823
dc.description.abstractThe epidermis is the external cell layer in direct contact with the environment, and it plays essential biological roles. Transcriptome analysis (RNA-seq) of Fragaria vesca fruit receptacles at four ripening stages (green, white, turning and red) and of different tissue types of receptacles (pith, vascular bundles, cortex and epidermis) at two ripening stages (green and red) allowed us to infer tissue- and stage-specific Gene Regulatory Networks (GRN). Due to the potential role of the epidermis in defense and in the differential anthocyanin accumulation pattern that shows at the ripe stage of F. vesca fruits (the skin is red, while the inner part is white), we have focused on the GRN of the ripe epidermis. In this study, we aim at the functional characterization of two transcription factors (TFs) that constituted the main hubs of this GRN: a MYB-like gene, and a member of the NAC family of TFs. A MapMan analysis of the genes constituting the GRN in ripe epidermis showed that wax and flavonoid biosynthesis were significantly overrepresented functions in this tissue at the ripe stage. Using the Luciferase/Renilla (Luc/Ren) system, the interaction of the MYB and NAC TFs with their wax-related putative targets was validated. To gain insight into the target genes of these two TFs, we mapped the genome-wide binding sites using DAP-seq analyses. Consistently, MYB bound to a set of genes involved in cuticle formation and flavonoid biosynthesis, while a number of genes involved in solute transport were enriched among the NAC targets. Currently, we are generating CRISPR/Cas9 mutant lines to functionally characterize these two TFs. Furthermore, we are performing protein interaction assays to decipher whether the MYB and NAC TFs interact with each other and with other TFs from the red epidermis GRN.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectFresases_ES
dc.subjectEpidermises_ES
dc.subject.otherStrawberryes_ES
dc.subject.otherTranscription Factores_ES
dc.titleCharacterization of ripe fruit epidermis-specific transcription factors in strawberryes_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle10th Rosaceae Genomics Conference - Virtual 2020es_ES
dc.relation.eventplaceCongreso virtuales_ES
dc.relation.eventdateDiciembre 2020es_ES
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*


Ficheros en el ítem

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional