Biología Molecular y Bioquímica - (BMB)
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The role of GDP-L-galactose phosphorylase in the control of ascorbate biosynthesis
Fenech, Mario; Amorim-Silva, Vitor; Esteban del Valle, Alicia; Arnoud, Dominique; Ruiz-López, Noemí
; Castillo-Garriga, Araceli; Smirnoff, Nicholas; Botella-Mesa, Miguel Ángel[et al.] (Oxford University Press in behalf of American Society of Plant Biologists, 2021-01-25)
The enzymes involved in l-ascorbate biosynthesis in photosynthetic organisms (the Smirnoff–Wheeler [SW] pathway) are well established. Here, we analyzed their subcellular localizations and potential physical interactions ... -
Postharvest changes in LIN5-down-regulated plants suggest a role for sugar deficiency in cuticle metabolism during ripening.
Vallarino Castro, José G.; Yeats, Trevor H.; Maximova, Eugenia; Rose, Jocelyn K.; Fernie, Alisdair R.; Osorio-Algar, Sonia[et al.] (Elsevier, 2017)
The cell wall invertase gene (LIN5) was reported to be a key enzyme influencing sugar uptake of tomato (Solanum lycopersicum) fruit. It was additionally revealed to be a key regulator of total soluble solids content in ... -
Characterisation of non-coding genetic variation in histamine receptors using AnNCR-SNP.
Rojano, Elena; García-Ranea, Juan Antonio; Perkins, James Richard (Springer, 2016-06-06)
Almost 90 % of disease-associated genetic variants found using genome wide association studies (GWAS) are located in non-coding regions of the genome. Such variants can affect phenotype by altering important regulatory ... -
Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress
Ruiz-López, Noemí; Pérez-Sancho, Jessica; Esteban del Valle, Alicia; Haslam, Richard P.; Vanneste, Steffen; Catalá, Rafael; Perea-Resa, Carlos; Van Damme, Daniël; García-Hernández, Selene; Albert, Armando; Vallarino, José; Lin, Jinxing; Friml, Jiří; Macho, Alberto P; Salinas, Julio; Rosado, Abel; Napier, Johnathan A.; Amorim-Silva, Vitor; Botella-Mesa, Miguel Ángel[et al.] (Oxford University Press in behalf of American Society of Plant Biologists, 2021-05-04)
Endoplasmic reticulum–plasma membrane contact sites (ER–PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER–PM protein tether synaptotagmin1 (SYT1) exhibit decreased PM integrity ... -
Ethylene is involved in strawberry fruit ripening in an organ-specific manner.
Merchante, Catharina; Valpuesta, Victoriano; Vallarino, José G.; Osorio-Algar, Sonia; Aragüez, Irene; Villareal, Natalia; Ariza, Maria T.; Martínez, Gustavo A.; Medina-Escobar, Nieves; Civello, Marcos P.; Fernie, Alisdair R.; Botella-Mesa, Miguel Ángel[et al.] (Oxford Academic, 2013-10-05)
The fruit of the strawberry F.ananassa has traditionally been classified as non-climacteric because its ripen- ing process is not governed by ethylene. However, previous studies have reported the timely endogenous ... -
Peripheral membrane proteins modulate stress tolerance by safeguarding cellulose synthases
Kesten, Christopher; García-Moreno, Álvaro; Amorim-Silva, Vitor; Menna, Alexandra; Castillo-Garriga, Araceli; Percio, Francisco; Armengot, Laia; Ruiz-López, Noemí; Jaillais, Yvon; Sánchez-Rodríguez, Clara; Botella-Mesa, Miguel Ángel[et al.] (American Association for the Advancement of Science, 2022-11-16)
Controlled primary cell wall remodeling allows plant growth under stressful conditions, but how these changes are conveyed to adjust cellulose synthesis is not understood. Here, we identify the TETRATRICOPEPTIDE THIOREDOXIN-LIKE ... -
Alteration of the Interconversion of Pyruvate and Malate in the Plastid or Cytosol of Ripening Tomato Fruit Invokes Diverse Consequences on Sugar But Similar Effects on Cellular Organic Acid, Metabolism, and Transitory Starch Accumulation.
Osorio-Algar, Sonia; Vallarino, José G.; Szecowka, Marek; Ufaz, Shai; Tzin, Vered; Angelovici, Ruthie; Galili, Gad; Fernie, Alisdair R.[et al.] (Oxford Academic, 2012-12-18)
The aim of this work was to investigate the effect of decreased cytosolic phosphoenolpyruvate carboxykinase (PEPCK) and plastidic NADP-dependent malic enzyme (NADP-ME) on tomato (Solanum lycopersicum) ripening. Transgenic ... -
CXCL5 mediates UVB irradiation-induced pain.
(AAAS, 2011-07-06)Many persistent pain states (pain lasting for hours, days, or longer) are poorly treated because of the limitations of existing therapies. Analgesics such as nonsteroidal anti-inflammatory drugs and opioids often provide ... -
Abiotic stresses differentially affect the expression of O-methyltransferase genes related to methoxypyrazine biosynthesis in seeded and parthenocarpic fruits of Vitis vinifera (L.)
(Elsevier, 2014-01-08)MPs (3-alkyl-2-methoxypyrazines) are grape-derived aroma compounds that are associated with detrimental herbaceous flavours in some wines. It is well known that several viticultural and environmental parameters can modulate ... -
A comparison of RNA-seq and exon arrays for whole genome transcription profiling of the L5 spinal nerve transection model of neuropathic pain in the rat
(SAGE Publications, 2014-01-28)The past decade has seen an abundance of transcriptional profiling studies of preclinical models of persistent pain, predominantly employing microarray technology. In this study we directly compare exon microarrays to ... -
Genetic diversity of strawberry germplasm using metabolomic biomarkers.
Vallarino, José G.; De Abreu e Lima, Francisco; Soria, Carmen; Tong, Hao; Pott, Delphine M.; Willmitzer, Lothar; Fernie, Alisdair R.; Nikoloski, Zoran; Osorio-Algar, Sonia[et al.] (Springer Nature, 2018-09-26)
High-throughput metabolomics technologies can provide the quantification of metabolites levels across various biological processes in different tissues, organs and species, allowing the identification of genes underpinning ... -
Gene3D: a domain-based resource for comparative genomics, functional annotation and protein network analysis
(Oxford University Press, 2012-01)Gene3D http://gene3d.biochem.ucl.ac.uk is a comprehensive database of protein domain assignments for sequences from the major sequence databases. Domains are directly mapped from structures in the CATH database or predicted ... -
Multi‐gene metabolic engineering of tomato plants results in increased fruit yield up to 23%.
(Springer Nature, 2020-10-14)The capacity to assimilate carbon and nitrogen, to transport the resultant sugars and amino acids to sink tissues, and to convert the incoming sugars and amino acids into storage compounds in the sink tissues, are key ... -
Transient protein-protein interactions: structural, functional, and network properties
(Elsevier, 2010-10-13)Transient interactions, which involve protein interactions that are formed and broken easily, are important in many aspects of cellular function. Here we describe structural and functional properties of transient interactions ... -
ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data
(BMC, 2012-07)Background: Measuring gene transcription using real-time reverse transcription polymerase chain reaction (RT-qPCR) technology is a mainstay of molecular biology. Technologies now exist to measure the abundance of many ... -
Flux balance analysis of metabolism during growth by osmotic cell expansion and its application to tomato fruits
(Wiley, 2020-01-27)Cell expansion is a significant contributor to organ growth and is driven by the accumulation of osmolytes to increase cell turgor pressure. Metabolic modelling has the potential to provide insights into the processes that ... -
From central to specialized metabolism: an overview of some secondary compounds derived from the primary metabolism for their role in conferring nutritional and organoleptic characteristics to fruit.
Pott, Delphine M.; Osorio-Algar, Sonia; Vallarino, José G. (Frontiers, 2019-06-28)
Fruit flavor and nutritional characteristics are key quality traits and ones of the main factors influencing consumer preference. Central carbon metabolism, also known as primary metabolism, contributes to the synthesis ... -
Regulatory variants: from detection to predicting impact.
(Oxford Academic, 2018-06-08)Variants within non-coding genomic regions can greatly affect disease. In recent years, increasing focus has been given to these variants, and how they can alter regulatory elements, such as enhancers, transcription factor ... -
Transcriptional profiling of dendritic cells in a mouse model of food-antigen induced anaphylaxis reveals the upregulation of multiple immune-related pathways.
Rodríguez, María José; Palomares, Francisca; Bogas, Gador; Torres-Jaén, María Josefa; Díaz-Perales, Araceli; Rojo, Javier; Plaza Serón, María del Carmen; Rodríguez-Nogales, Alba; Orengo, Christine A.; Mayorga, Cristobalina; Perkins, James Richard[et al.] (Wiley, 2018-10-20)
Scope Much of our knowledge about gene expression during anaphylaxis comes from candidate gene studies. Despite their potential role, expression changes in dendritic cells (DCs) have not been studied in this context using ... -
Targeting VEGFR-3/-2 signaling pathways with AD0157: a potential strategy against tumor-associated lymphangiogenesis and lymphatic metastases
(BMC, 2017-06-19)La metástasis linfática es una de las principales causas de muerte en pacientes con diferentes tipos de cáncer y es el principal factor pronóstico para la supervivencia de la enfermedad. La formación de nuevos vasos ...