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dc.contributor.authorSánchez-Gómez, Carlos
dc.contributor.authorMeco, Victoriano
dc.contributor.authorUrrutia, María
dc.contributor.authorCastillo-Garriga, Araceli 
dc.contributor.authorPérez-Sancho, Jessica
dc.contributor.authorBayer, Emmanuelle M.
dc.contributor.authorFranco-Zorrila, José Manuel
dc.contributor.authorMartín-Pizarro, Carmen
dc.contributor.authorPose-Padilla, David
dc.date.accessioned2022-01-11T13:36:58Z
dc.date.available2022-01-11T13:36:58Z
dc.date.issued2021-09-13
dc.identifier.urihttps://hdl.handle.net/10630/23569
dc.description.abstractThe epidermis is the external cell layer in direct contact with the environment, and it plays essential biological roles. Transcriptome analysis (RNA-seq) of Fragaria vesca fruit receptacles at four ripening stages and of different tissue types of receptacles (pith, vascular bundles, cortex and epidermis) at two ripening stages (green and red) allowed us to infer tissue- and stage-specific Gene Regulatory Networks (GRN). Due to the potential role of the epidermis in defense and in the differential anthocyanin accumulation pattern that shows F. vesca fruits at the ripe stage, we have focused on the GRN of the epidermis of red receptacles. In this study, we aim at the functional characterization of two transcription factors (TFs) that constituted the main hubs: a MYB-like gene, and a member of the NAC family of TFs. MapMan analysis of the GRN showed that wax and flavonoid biosynthesis were significantly overrepresented functions. Using the Luciferase/Renilla system, the interaction of the MYB and NAC TFs with their wax-related putative targets was validated. To experimentally validate the target genes of these two TFs, their binding sites were mapped genome-wise using DAP-seq analyses. Consistently, MYB bound to genes involved in cuticle formation and flavonoid biosynthesis, while genes involved in solute transport were enriched among NAC targets. Finally, protein interaction assays showed that MYB physically interacts with two members of the bHLH family specifically expressed in the red epidermis, leading to the subcellular relocalization of one of them from the cytoplasm to the nucleus.es_ES
dc.description.sponsorshipPlan Estatal de Investigación Científica y Técnica y de Innovación RTI2018-097309-A-I00 and the ERC Starting Grant ERC-2014-StG 638134es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectFresases_ES
dc.subject.otherStrawberryes_ES
dc.subject.otherTranscription Factores_ES
dc.subject.otherEpidermises_ES
dc.titleCharacterization of ripe fruit epidermis-specific transcription factors in strawberryes_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXXXIII Argentinian Meeting of Plant Physiology (RAFV 2021) - edición onlinees_ES
dc.relation.eventplaceOnlinees_ES
dc.relation.eventdateSeptiembre 2021es_ES
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*


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