- RIUMA Principal
- Investigación
- Microbiología - (MIC)
- Listar Microbiología - (MIC) por autor
Listar Microbiología - (MIC) por autor "Alonso-Sánchez, María del Carmen"
Mostrando ítems 1-20 de 30
-
Antiviral activity of European sea bass ISG15 against betanodavirus infections
Moreno, Patricia; García-Rosado, Esther; Borrego-García, Juan José; Alonso-Sánchez, María del Carmen (2017)To study the role of the sea bass ISG15 protein against betanodavirus infections, an in vivo transcription analysis has been performed after RGNNV infection and poly I:C inoculation, and the sea bass ISG15 activity has ... -
Betanodavirus virulence to European sea bass (Dicentrarchus labrax): a new insight into virus-host interaction
Moreno Garcia, Patricia (UMA Editorial, 2018-12-21)Viral diseases are the result of the pathogen virulence, the host immunity mechanisms, and the interaction between those components, which constitutes the so called host-pathogen interaction. This PhD Thesis is a comprehensive ... -
Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass.
Moreno, Patricia; Souto, Sandra; Leiva-Rebollo, Rocio; Borrego-García, Juan José; Bandin, Isabel; Alonso-Sánchez, María del Carmen[et al.] (Nature Pubishing Group, 2019-10-01)The role of the capsid protein (Cp) amino acids 247 and 270 in the virulence of a RGNNV isolate to sea bass (Dicentrarchus labrax) has been evaluated by the generation of recombinant RGNNV viruses harbouring SJNNV-type ... -
Characterization of Senegalese sole (Solea senegalensis) Mx promoter
Carballo Perez, Carlos; Alonso-Sánchez, María del Carmen; Collet, Bertrand; Béjar-Alvarado, Julia; Álvarez-Torres, Daniel; García-Rosado, Esther[et al.] (Elsevier, 2013)Type I interferon (IFN) represents a first component of the innate immune response against viral infections, promoting an antiviral state in cells. Mx proteins are the best studied IFN-inducible genes (ISGs) in fish. The ... -
Cytokine-like activity of European sea bass ISG15 protein on RGNNV-infected E−11 cells
Moreno-García, Patricia; Leiva-Rebollo, Rocío; García-Rosado, Esther; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen (Elsevier, 2022-08-23)IFN-I generates an antiviral state by inducing the expression of numerous genes, called IFN-stimulated genes, ISGs, including ISG15, which is the only ISG with cytokine-like activity. In a previous study, we developed ... -
Differences in the viral induction of the senegalese sole Mx protein in RTG-2 and CHSE-214 cells
Álvarez-Torres, Daniel; Podadera, Ana Maria; García-Rosado, Esther; Collet, Bertrand; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen[et al.] (EAFP, 2013)Transfected RTG-2 cells infected with VHSV showed significant induction of the luciferase reporter gene, compared to the control non-infected cells, at 24, 48 and 72 h post infection (p.i.). The maximum expression was ... -
Differential antiviral activity of European sea bass interferon-stimulated 15 protein (ISG15) against RGNNV and SJNNV betanodaviruses.
Moreno, Patricia; Álvarez-Torres, Daniel; García-Rosado, Esther; Borrego-García, Juan José; Alonso-Sánchez, María del Carmen (ScienceDirect, 2018-09-07)ISG15 is an antiviral protein acting intracellularly, by conjugation to viral or cellular proteins, or extracellularly, as cytokine. In this work, an in vitro system, consisting of E-11 cells over-expressing European sea ... -
Differential antiviral activity of Porphyridium cruentum polysaccharides against VHSV and NNV infections
Parra-Riofrio, Geovanna; Moreno-García, Patricia; Abdala-Díaz, Roberto Teófilo; García-Rosado, Esther; Uribe-Tapia, Eduardo; Alonso-Sánchez, María del Carmen; Béjar-Alvarado, Julia[et al.] (2021-09)The use of functional food can be an interesting approach to prevent viral diseases in aquaculture facilities. An increasing number of studies have suggested that marine microorganisms can be a relevant source of substances ... -
Differential immunogene expression profile of European sea bass (Dicentrarchus labrax, L.) in response to highly and low virulent NN
Moreno, Patricia; Gémez-Mata, Juan; García-Rosado, Esther; Béjar-Alvarado, Julia; Labella, Alejandro M; Souto, Sandra; Alonso-Sánchez, María del Carmen[et al.] (Elsevier, 2020)European sea bass is highly susceptible to the nervous necrosis virus, RGNNV genotype, whereas natural outbreaks caused by the SJNNV genotype have not been recorded. The onset and severity of an infectious disease depend ... -
Estimulación del sistema mediado por interferón tipo I de lenguado senegalés (Solea senegalensis) en respuesta a infecciones por nodavirus
Podadera, AM; Álvarez-Torres, Daniel; Souto, S; Bandín Matos, Isabel; Alonso-Sánchez, María del Carmen (2014-09-25)El lenguado senegalés es susceptible a la infección por el Virus de la Necrosis Nerviosa Viral (VNNV). Los betanodavirus se clasifican en cuatro genotipos, siendo los genotiposSJNNV, RGNNV y virus recombinantes RGNNV-SJNNV ... -
Estudio in vitro de la actividad antagonista de aislados del virus de la septicemia hemorrágica viral (VHSV) de distinto origen sobre el sistema del IFN tipo I
Gémez-Mata, Juan; Álvarez-Torres, Daniel; Alonso-Sánchez, María del Carmen; García-Rosado, Esther; Béjar-Alvarado, Julia (2018-11-09)El lenguado senegalés (Solea senegalensis) es susceptible a aislados marinos del virus de la septicemia hemorrágica viral (VHSV); sin embargo, los VHSV patógenos para especies de agua dulce no son virulentos para especies ... -
Estudio in vitro de la actividad tipo citoquina de ISG15 de lubina frente a infecciones por Betanodavirus
Moreno-García, Patricia; Leiva-Rebollo, Rocío; García-Rosado, Esther; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen (2022-09)El sistema interferón tipo I (IFN-I) establece un estado antiviral mediante la transcripción de los llamados genes estimulados por IFN (ISG). Destaca el gen isg15, cuya proteína es la única descrita que presenta actividad ... -
European sea bass RTP3 genes: genomic characterization and transcription analyses.
Moreno-García, Patricia; Gémez-Mata, Juan; Álvarez-Torres, Daniel; García-Rosado, Esther; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen[et al.] (2023)Introduction: Fish RTP3, belonging to receptor-transporting protein (RTP) family, has been described as an interferon-α (IFN-α)-responsive gene. However, little information is available about fish rtp3 gene structure and ... -
Extractos del probiótico Shewanella putrefaciens Pdp11 protegen frente a la infección por RGNNV en cultivo celular
Moreno, Patricia; Balebona-Accino, María del Carmen; Moriñigo-Gutiérrez, Miguel Ángel; Alonso-Sánchez, María del Carmen; Béjar-Alvarado, Julia; García-Rosado, Esther[et al.] (2022)El agente etiológico de la retinopatía y encefalitis viral (VER) es el virus de la necrosis nerviosa (VNN). Aunque esta enfermedad puede causar una elevada mortalidad en numerosas especies de peces, no existen métodos ... -
Genomic characterization and transcription analysis of European sea bass (Dicentrarchus labrax) rtp3 genes
Moreno-García, Patricia; Gémez-Mata, Juan; Álvarez-Torres, Daniel; García-Rosado, Esther; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen[et al.] (Elsevier, 2023-10-23)Fish RTP3, belonging to the receptor-transporting protein family, display several functions, including a putative antiviral role as virus-responsive gene. In this work, we have identified and characterized two different ... -
Genomic characterization and transcription analysis of European sea bass (Dicentrarchus labrax) rtp3 genes.
Moreno, Patricia; Gémez-Mata, Juan; Álvarez-Torres, Daniel; García-Rosado, Esther; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen[et al.] (Elsevier, 2023-10-23)Fish RTP3, belonging to the receptor-transporting protein family, display several functions, including a putative antiviral role as virus-responsive gene. In this work, we have identified and characterized two different ... -
In vitro viral coexistence decreases Striped Jack Nervous Necrosis Virus (SJNNV) but favours Red-spotted Grouper Nervous Necrosis Virus (RGNNV) replication
López-Jimena, Benjamín; Alonso-Sánchez, María del Carmen; Infante, Carlos; Castro-López, María Dolores; Borrego-García, Juan José; García-Rosado, Esther[et al.] (European Association of Fish Pathologists, 2013)In this study, several assays have been performed to characterise the effect of the RGNNV-SJNNV coexistence (coinfection and superinfection) on the replication of each genotype on E-11 cells.The results obtained showed the ... -
Infection of Artemia sp. by Lymphocystis disease virus (LCDV)
Jiménez Valverde, Estefanía; Cano, Irene; García-Rosado, Esther; Alonso-Sánchez, María del Carmen; Borrego-García, Juan José; Castro-López, María Dolores[et al.] (2013-12-10)In the present work, different developmental stages of Artemia sp. (metanauplius, juvenile and adult) were experimentally infected with LCDV by immersion. Results of viral quantification (both by qPCR and cell-culture viral ... -
Inhibition of nervous necrosis virus replication by Shewanella putrefaciens Pdp11 extract
Moreno, Patricia; Álvarez-Torres, Daniel; Balebona-Accino, María del Carmen; Domínguez-Maqueda, Marta; Moriñigo-Gutiérrez, Miguel Ángel; Béjar-Alvarado, Julia; Alonso-Sánchez, María del Carmen; García-Rosado, Esther[et al.] (Elsevier, 2023)One of the most relevant infectious diseases affecting farmed fish is the viral encephalopathy and retinopathy, caused by the nervous necrosis virus (NNV). The two commercial vaccines available against this virus have been ... -
Papel del inmunogen isg15 de lubina (Dicentrarchus labrax) en las infecciones por betanodavirus
Moreno, Patricia; García-Rosado, Esther; Borrego-García, Juan José; Alonso-Sánchez, María del Carmen (Universidad de Oviedo, 2016)En peces, al igual que en vertebrados superiores, la respuesta antiviral innata está mediada por el sistema interferón tipo I (IFN I), que actúa mediante la activación de genes denominados isg (interferon-stimulated genes), ...