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dc.contributor.authorGémez-Mata, Juan
dc.contributor.authorLeiva-Rebollo, Rocío
dc.contributor.authorMoreno-García, Patricia
dc.contributor.authorBandín Matos, Isabel
dc.contributor.authorBorrego-García, Juan José 
dc.contributor.authorLabella Vera, Alejandro Manuel
dc.contributor.authorCastro-López, María Dolores 
dc.date.accessioned2022-09-14T09:18:40Z
dc.date.available2022-09-14T09:18:40Z
dc.date.issued2022-09
dc.identifier.urihttps://hdl.handle.net/10630/24985
dc.description.abstractViral nervous necrosis (VNN) is a disease that affects farmed fish worldwide. Its etiologic agent is the nervous necrosis virus (NNV), genus Betanodavirus, family Nodaviridae. NNV are small and non-enveloped viruses with a genome consisting of two molecules of positive-sense single-stranded RNA, RNA1 and RNA2, which encode the RNA-dependent RNA polymerase and the capsid protein, respectively. The betanodaviruses have been classified into four species: Striped jack nervous necrosis virus (SJNNV), Tiger puffer nervous necrosis virus (TPNNV), Red-spotted grouper nervous necrosis virus (RGNNV), and Barfin flounder nervous necrosis virus (BFNNV). In Southern Europe, natural reassortants between RGNNV and SJNNV have been isolated from Senegalese sole (Solea senegalensis), gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) associated to VNN outbreaks. Immune response against betanodavirus infections has been poorly studied in gilthead seabream. In this study, fish were challenged by intramuscular (im) injection or by immersion, using a reassortant strain containing RGNNV-type RNA1 and SJNNV-type RNA2 segments. Head kidney and brain samples were collected at 24, 48 and 72 h post-challenge (pc) for the injection experiment, while in the bath challenge sampling was performed at 48 and 72 h pc. The immunogen expression analysis was carried out using the platform OpenArray®. In the im-injected fish, 21 differentially expressed genes (DEGs) were identified in head kidney samples at 24 h pc, whereas a lower immune response was detected at 48 and 72 h pc (11 and 9 DEGs, respectively). In brain samples, a delayed response was observed, with 32 DEGs recorded at 72 h pc. Regarding the bath-challenged fish, fewer immunogenes were differentially expressed although all of them were up-regulated. This research was funded by the Ministerio de Ciencia, Innovación y Universidades (MCIUI) and FEDER under Grant RTI2018-094687-B.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Teches_ES
dc.language.isospaes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectVacunación de animales - Congresoses_ES
dc.subjectDoradas - Enfermedades por virus - Congresoses_ES
dc.subjectVacunas ADN - Congresoses_ES
dc.subjectNervios - Necrosis - Congresoses_ES
dc.subject.otherNervous necrosis viruses_ES
dc.subject.otherGilthead seabreames_ES
dc.subject.otherImmune gene expressiones_ES
dc.titleImmune gene expression in gilthead seabream after nervous necrosis virus (NNV) challengees_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXVI Congreso Nacional de Virologíaes_ES
dc.relation.eventplaceMálaga, Españaes_ES
dc.relation.eventdate6 septiembre 2022es_ES


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