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dc.contributor.authorÁlvarez-Mena, Ana
dc.contributor.authorDíaz-Martínez, Luis
dc.contributor.authorCaro-Astorga, Joaquín
dc.contributor.authorKuipers, Oscar
dc.contributor.authorDe-Vicente-Moreno, Antonio 
dc.contributor.authorRomero-Hinojosa, Diego Francisco 
dc.date.accessioned2022-09-25T05:58:52Z
dc.date.available2022-09-25T05:58:52Z
dc.date.created2022
dc.date.issued2022
dc.identifier.urihttps://hdl.handle.net/10630/25067
dc.description.abstractThe human pathogen Bacillus cereus is responsible of many food poisoning due to the ingestion of contaminated vegetables or processed foods. For the survival and colonization, the bacteria form a biofilm on biotic and abiotic surfaces in which a specific subpopulation differentiates to produce a multifunctional extracellular matrix (ECM), mainly composed by exopolysaccharides, proteins and extracellular DNA. One of the most attractive components of the ECM are the amyloid proteins due to its high tendency to fibrillate and the multifunctional role in the bacterial physiology. In the B. cereus genome there is a specific genomic region that contains two orthologues genes to the tasA gene in Bacillus subtilis, initially described as essential in the assembly of amyloid fibers, named tasA and calY respectively. The deletion of these genes gives a different phenotype related with a deficient biofilm formation in both cases. In the tasA mutant, the biofilm finally detaches at 72 hours, and the calY mutant shows a thinner ring phenotype in comparison with the wild type strain. The gene expression dynamic during the biofilm development reveals that there are three different subpopulations: both genes are expressed, only calY or none of them. Furthermore, transcriptomic analysis by RNA-Seq show that the mutants have different changes in the gene regulation and the calY mutant is the most affected, with 227 genes deregulated that affect to several important pathways such as the synthesis of extracellular DNA, an important component of the ECM. The results mentioned above indicate that TasA and CalY might have complementary roles in the biopellicle development but also evidence that this physiological process is more complex in B. cereus in comparison with the model described in B. subtilis.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isospaes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectAmiloidees_ES
dc.subjectBacillus (Bacteria)es_ES
dc.subject.otherBacillus cereuses_ES
dc.subject.otherAmyloides_ES
dc.subject.otherBiofilmes_ES
dc.titleThe different roles of TasA and CalY in the biofilm formation of B. cereuses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXIII Reunión de grupo especializado microbiología moleculares_ES
dc.relation.eventplaceGranadaes_ES
dc.relation.eventdate7 Septiembre 2022es_ES


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