Botrytis cinerea, the causal agent of the gray mold disease, is one of the main limiting factors of
horticultural crops production worldwide, consuming up to 40% of fungicides in its control.
However, this fungus has been categorized by FRAC (Fungicide Resistance Action Committee)
as a phytopathogen with a high risk for fungicide resistance development, a fact that has been
demonstrated in our country. In addition, and according to the "farm to fork" strategy of the
recent European Green Deal, the diversity of fungicides available to growers will be reduced by
50% in 2030. For this reason, alternative control tools and molecules with fungicide activity are,
more than ever, needed to control this important disease. In this study, we intend to check if the
efficacy of the emerging RNA interference (RNAi) strategy, called "spray-induced gene
silencing" (SIGS), could be a valid sustainable solution and an alternative to the use of
conventional fungicides for the control of B. cinerea. For this purpose, several double-stranded
RNA (dsRNA) has been designed against targets genes involved in the virulence/pathogenicity
of the fungus. Preliminary results, obtained in in vivo assays, indicated that the application of
dsRNAs significantly reduces the development of the fungus, demonstrating the potential of the
SIGS technique for the control of B. cinerea. On the other hand, and to improve the application
of these oligonucleotides in the field, their encapsulation to create nanoparticles will be carried
out. If we succeed, new molecules with fungicidal action, could be included into the several
strategies carried out to obtain a sustainable plant protection control programs in the field
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