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dc.contributor.authorGarcía-Márquez, Jorge
dc.contributor.authorRodrigues Moreira, Bruna
dc.contributor.authorValverde-Guillén, Piedad
dc.contributor.authorLatorre-Redoli, Sofía
dc.contributor.authorCaneda-Santiago, Candela T.
dc.contributor.authorAcién-Fernández, Francisco Gabriel
dc.contributor.authorMartínez-Manzanares, Eduardo 
dc.contributor.authorMari-Beffa, Manuel 
dc.contributor.authorAbdala-Díaz, Roberto Teófilo 
dc.date.accessioned2023-06-19T07:43:34Z
dc.date.available2023-06-19T07:43:34Z
dc.date.created2023-06-19
dc.date.issued2023-04-28
dc.identifier.citationGarcía-Márquez J, Moreira BR, Valverde-Guillén P, Latorre-Redoli S, Caneda-Santiago CT, Acién G, Martínez-Manzanares E, Marí-Beffa M, Abdala-Díaz RT. In Vitro and In Vivo Effects of Ulvan Polysaccharides from Ulva rigida. Pharmaceuticals. 2023; 16(5):660. https://doi.org/10.3390/ph16050660es_ES
dc.identifier.urihttps://hdl.handle.net/10630/26991
dc.description.abstractOne of the main bioactive compounds of interest from the Ulva species is the sulfated polysaccharide ulvan, which has recently attracted attention for its anticancer properties. This study investigated the cytotoxic activity of ulvan polysaccharides obtained from Ulva rigida in the following scenarios: (i) in vitro against healthy and carcinogenic cell lines (1064sk (human fibroblasts), HACAT (immortalized human keratinocytes), U-937 (a human leukemia cell line), G-361 (a human malignant melanoma), and HCT-116 (a colon cancer cell line)) and (ii) in vivo against zebrafish embryos. Ulvan exhibited cytotoxic effects on the three human cancer cell lines tested. However, only HCT-116 demonstrated sufficient sensitivity to this ulvan to make it relevant as a potential anticancer treatment, presenting an LC50 of 0.1 mg mL−1. The in vivo assay on the zebrafish embryos showed a linear relationship between the polysaccharide concentration and growth retardation at 7.8 hpf mL mg−1, with an LC50 of about 5.2 mg mL−1 at 48 hpf. At concentrations near the LC50, toxic effects, such as pericardial edema or chorion lysis, could be found in the experimental larvae. Our in vitro study supports the potential use of polysaccharides extracted from U. rigida as candidates for treating human colon cancer. However, the in vivo assay on zebrafish indicated that the potential use of ulvan as a promising, safe compound should be limited to specific concentrations below 0.001 mg mL−1 since it revealed side effects on the embryonic growth rate and osmolar balance.es_ES
dc.description.sponsorshipPartial funding for open access charge: Universidad de Málagaes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCompuestos bioactivos vegetaleses_ES
dc.subjectAntineoplásicoses_ES
dc.subjectAlgas-Biotecnologíaes_ES
dc.subject.otherCytotoxic activityes_ES
dc.subject.otherHuman cancer cell lineses_ES
dc.subject.otherPolysaccharideses_ES
dc.subject.otherUlva rigidaes_ES
dc.subject.otherUlvanes_ES
dc.subject.otherZebrafish embryo toxicity testes_ES
dc.titleIn vitro and in vivo effects of ulvan polysaccharides from Ulva rigidaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.centroFacultad de Cienciases_ES
dc.identifier.doihttps://doi.org/10.3390/ph16050660
dc.rights.ccAtribución 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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