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dc.contributor.authorGonzález-García, Marta
dc.contributor.authorCarrillo-Franco, Laura
dc.contributor.authorMorales-Luque, Carmen
dc.contributor.authorDíaz-Casares, Amelia 
dc.contributor.authorPonce-Velasco, Marina
dc.contributor.authorGago-Calderón, Belén 
dc.contributor.authorDawid-Milner, Marc Stefan 
dc.contributor.authorLópez-González, Manuel Víctor 
dc.date.accessioned2023-07-28T11:21:27Z
dc.date.available2023-07-28T11:21:27Z
dc.date.issued2023-07
dc.identifier.urihttps://hdl.handle.net/10630/27391
dc.description.abstractThe stimulation of the Periaqueductal Gray matter (PAG) and nucleus retroambiguus (nRA) produces vocalization. A high expression of FOXP2 protein at mesencephalic (PAG) and pontine regions involved in cardiorespiratory control has been described. The aim of this study was to characterize the possible role of the dlPAG in modulating laryngeal activity and their effects on vocalization. Experimental studies were carried out with non-inbred male rats (n=27), Sprague-Dawley (250-300 g). Animals were anesthetized with sodium pentobarbitone (60 mg/kg i.p., initial dose, supplemented 2mg/ kg, i.v., as necessary). Neuromorphological study (n=6) The pattern of staining for c-Fos and FOXP2 protein immunoreactivity (c-Fos-ir) were examinated throughout the rostrocaudal extent of the nRa/nA region during electrical stimulation of the dlPAG. Neuropharmacological study (n=21) A double tracheal cannulation was used to obtain an “isolated glottis in situ” and to record respiratory airflow. Bilateral parietostomy allowed access to the dlPAG. Electrical stimulations (n=7) of this region using concentric bipolar electrodes (1ms pulses, 20-40µA, 100Hz for 5s) were performed. Microinjections of PBS-Evans Blue (250nl, pH 7.4±0.1, 5-s duration) (n=7) or glutamate (0,25M, 250nl) (n=7) were performed. Respiratory flow, pleural pressure, blood pressure and heart rate were also recorded. Activation of the dlPAG elicited a selective increase in c-Fos-ir with an ipsilateral predominance in nRA/nA somatas (p<0.01) and confirm the expression of FOXP2 bilaterally in both nuclei. dlPAG PBS-Evans Blue microinjections did not produce any significant changes in any of the cardiorespiratory variables recorded. dlPAG stimulations evoked a decrease of laryngeal resistance (subglottal pressure) (p<0,001) accompanied with an inspiratory facilitatory response consisted of an increase in respiratory rate (p<0,001), together with a pressor (p<0,001) and tachycardic response (p<0,001).es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectFisiología comparadaes_ES
dc.subject.otherSubglottic Pressurees_ES
dc.subject.otherLaryngeal Motoneuronses_ES
dc.subject.otherdlPAGes_ES
dc.subject.otherNucleus Ambiguuses_ES
dc.titleLaryngeal effects of stimulation of the dorsolateral Periaqueductal Grey Matter in spontaneously breathing anaesthetized ratses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Medicinaes_ES
dc.relation.eventtitlePHYSIOLOGY 2023es_ES
dc.relation.eventplaceHARROGATE, Reino Unidoes_ES
dc.relation.eventdate10-07-2023es_ES


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