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dc.contributor.authorMoreno, Patricia
dc.contributor.authorSouto, Sandra
dc.contributor.authorLeiva-Rebollo, Rocío
dc.contributor.authorBorrego-García, Juan José 
dc.contributor.authorBandin, Isabel
dc.contributor.authorAlonso-Sánchez, María del Carmen 
dc.date.accessioned2024-01-25T12:48:18Z
dc.date.available2024-01-25T12:48:18Z
dc.date.created2024
dc.date.issued2019-10-01
dc.identifier.citationMoreno, P., Souto, S., Leiva-Rebollo, R. et al. Capsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass. Sci Rep 9, 14068 (2019).es_ES
dc.identifier.urihttps://hdl.handle.net/10630/29225
dc.description.abstractThe role of the capsid protein (Cp) amino acids 247 and 270 in the virulence of a RGNNV isolate to sea bass (Dicentrarchus labrax) has been evaluated by the generation of recombinant RGNNV viruses harbouring SJNNV-type amino acids in the above mentioned positions (Mut247Dl965, Mut270Dl965 and Mut247+270Dl965). Viral in vitro and in vivo replication, virus virulence and fish immune response triggered by these viruses have been analysed. Mutated viruses replicate on E-11 cells, although showing some differences compared to the wild type virus, suggesting that the mutations can affect the viral cell recognition and entry. In vivo, fish mortality caused by mutated viruses was 60% lower, and viral replication in sea bass brain was altered compared to non-mutated virus. Regarding sea bass immune response, mutated viruses triggered a lower induction of IFN I system and inflammatory response-related genes. Furthermore, mutations caused changes in viral serological properties (especially the mutation in amino acid 270), inducing higher seroconversion and changing antigen recognition.es_ES
dc.description.sponsorshipThis study has been supported by the projects AGL2017-84644-R (MINECO/AEI/FEDER, UE) and AGL2014-53532-C (MINECO/FEDER).es_ES
dc.language.isoenges_ES
dc.publisherNature Pubishing Groupes_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLubinas - Viruses_ES
dc.subjectAminoácidoses_ES
dc.subject.otherEuropean sea basses_ES
dc.subject.otherVirulencees_ES
dc.subject.otherReverse geneticses_ES
dc.subject.otherImmune responsees_ES
dc.subject.otherBetanodaviruses_ES
dc.titleCapsid amino acids at positions 247 and 270 are involved in the virulence of betanodaviruses to European sea bass.es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.centroFacultad de Cienciases_ES
dc.identifier.doi10.1038/s41598-019-50622-1
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/submittedVersiones_ES


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