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dc.contributor.authorCórdoba Caballero, José
dc.contributor.authorSeoane, Pedro
dc.contributor.authorJabato, Fernando M.
dc.contributor.authorPerkins, James R.
dc.contributor.authorManchado, Manuel
dc.contributor.authorClaros, Manuel G.
dc.date.accessioned2024-01-26T13:19:17Z
dc.date.available2024-01-26T13:19:17Z
dc.date.issued2020
dc.identifier.citationCórdoba-Caballero, J., Seoane, P., Jabato, F.M. et al. An improved de novo assembling and polishing of Solea senegalensis transcriptome shed light on retinoic acid signalling in larvae. Sci Rep 10, 20654 (2020). https://doi.org/10.1038/s41598-020-77201-zes_ES
dc.identifier.urihttps://hdl.handle.net/10630/29336
dc.description.abstractSenegalese sole is an economically important species in aquaculture and a model to decipher the severe transformations occurring during flatfish metamorphosis. A robust sole transcriptome was envisaged from a total of 96 resulting assemblies to provide SOLSEv5.0, the most informative transcriptome with low redundancy and devoid of most single-exon transcripts. It included both Illumina and GS-FLX reads and consisted of 51,348 transcripts of which 22,684 code for 17,429 different proteins described in databases, where 9527 were predicted as complete proteins. SOLSEv5.0 was used as reference for the study of retinoic acid (RA) signalling in sole larvae using drug treatments (DEAB, a RA synthesis blocker, and TTNPB, a RA-receptor agonist) for 24 and 48 h. Differential expression and functional interpretation were facilitated by an updated version of DEGenes Hunter. Acute exposure of both drugs triggered an intense, specific and transient response at 24 h but with hardly observable differences after 48 h at least in the DEAB treatments. Activation of RA signalling by TTNPB specifically increased the expression of genes in pathways related to RA degradation, retinol storage, carotenoid metabolism, homeostatic response and visual cycle, and also modified the expression of transcripts related to morphogenesis and collagen fibril organisation. In contrast, DEAB mainly decreased genes related to retinal production, impairing phototransduction signalling in the retina. A total of 755 transcripts mainly related to lipid metabolism, lipid transport and lipid homeostasis were altered in response to both treatments, indicating non-specific drug responses associated with intestinal absorption. These results indicate that a new assembling and transcript sieving were both necessary to provide a reliable transcriptome to identify the many aspects of RA action during sole development that are of relevance for sole aquaculture.es_ES
dc.language.isoenges_ES
dc.publisherScientific Reports (Springer Nature)es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLenguados - Cría y explotaciónes_ES
dc.subjectAcuiculturaes_ES
dc.subject.otherDe novo assemblinges_ES
dc.subject.otherTranscriptomees_ES
dc.subject.otherSenegalese solees_ES
dc.subject.otherRetinoic acides_ES
dc.titleAn improved de novo assembling and polishing of Solea senegalensis transcriptome shed light on retinoic acid signalling in larvaees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.centroFacultad de Cienciases_ES
dc.identifier.doi10.1038/s41598-020-77201-z
dc.rights.ccAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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