This study aimed to assess hand function using surface electromyography and accelerometer-type inertial sensors. Six participants from a healthy population performed the extension grip while their movements were analyzed and recorded. The data analysis revealed five phases within the movement based on the vector module of the index finger and electromyography of the first dorsal interosseous musculature. This real-time parameterization of the extension grip provided new variables for analysis and complemented standardized assessments of hand function. The use of these devices in a synchronous manner allows clinicians to identify new variables for intervention and treatment. The study also found that muscles in the hypothenar region and the first dorsal interosseous were more activated during grip extension, while the wrist extensor muscles and the ulnar flexor carpi ulnaris were more activated during the grip approach phase. The variation of acceleration could indicate abnormal movements or tremors. Overall, this protocol offers a noninvasive and harmless approach to assessing hand function and can guide rehabilitation efforts.