The use of natural sounds in auditory brain-computer interfaces (BCI) has been shown to improve classification results and usability. Some auditory BCIs are based on stream segregation, in which the subjects must attend one audio stream and ignore the other(s); these streams include some kind of stimuli to be detected. In this work we focus on event-related potentials (ERP) and study whether providing intelligible content to each audio stream could help the users to better concentrate on the desired stream and so to better attend the target stimuli and to ignore the non-target ones. Approach. In addition to a control condition, two experimental conditions, based on the selective attention and the cocktail party effect, were tested using two simultaneous and spatialized audio streams: i) the condition A2 consisted of an overlap of auditory stimuli (single syllables) on a background consisting of natural speech for each stream, ii) in condition A3, brief alterations of the natural flow of each speech were used as stimuli. Main results. The two experimental proposals improved the results of the control condition (single words as stimuli without a speech background) both in a cross validation analysis of the calibration part and in the online test. The analysis of the ERP responses also presented better discriminability for the two proposals in comparison to the control condition. The results of subjective questionnaires support the better usability of the first experimental condition. Significance. The use of natural speech as background improves the stream segregation in an ERP-based auditory BCI (with significant results in the performance metrics, the ERP waveforms, and in the preference parameter in subjective questionnaires). Future work in the field of ERP-based stream segregation should study the use of natural speech in combination with easily perceived but not distracting stimuli.
