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dc.contributor.authorMolina-Santiago, Carlos
dc.contributor.authorPearson, John
dc.contributor.authorBerlanga-Clavero, María Victoria
dc.contributor.authorPérez-Lorente, Alicia Isabel
dc.contributor.authorDe-Vicente-Moreno, Antonio 
dc.contributor.authorRomero-Hinojosa, Diego Francisco 
dc.date.accessioned2024-09-30T10:14:39Z
dc.date.available2024-09-30T10:14:39Z
dc.date.issued2022-07-05
dc.identifier.citationMolina-Santiago C, Pearson JR, Berlanga-Clavero MV, Pérez-Lorente AI, de Vicente A, Romero D. A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics. Microbiol Spectr. 2022 Aug 31;10(4):e0093922. doi: 10.1128/spectrum.00939-22. Epub 2022 Jul 5. PMID: 35862940; PMCID: PMC9430563.es_ES
dc.identifier.urihttps://hdl.handle.net/10630/33999
dc.description.abstractComplex interactions between microbial populations can greatly affect the overall properties of a microbial community, sometimes leading to cooperation and mutually beneficial coexistence, or competition and the death or displacement of organisms or subpopulations. Interactions between different biofilm populations are highly relevant in diverse scientific areas, from antimicrobial resistance to microbial ecology. The utilization of modern microscopic techniques has provided a new and interesting insight into how bacteria interact at the cellular level to form and maintain microbial biofilms. However, our ability to follow complex intraspecies and interspecies interactions in vivo at the microscopic level has remained somewhat limited. Here, we detailed BacLive, a novel noninvasive method for tracking bacterial growth and biofilm dynamics using high-resolution fluorescence microscopy and an associated ImageJ processing macro (https://github.com/BacLive) for easier data handling and image analysis. Finally, we provided examples of how BacLive can be used in the analysis of complex bacterial communities. IMPORTANCE Communication and interactions between single cells are continuously defining the structure and composition of microbial communities temporally and spatially. Methods routinely used to study these communities at the cellular level rely on sample manipulation which makes microscopic time-lapse experiments impossible. BacLive was conceived as a method for the noninvasive study of the formation and development of bacterial communities, such as biofilms, and the formation dynamics of specialized subpopulations in time-lapse experiments at a colony level. In addition, we developed a tool to simplify the processing and analysis of the data generated by this method.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiology (ASM)es_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectMicroscopíaes_ES
dc.subjectBacteriases_ES
dc.subject.otherMicroscopyes_ES
dc.subject.otherBacteriaes_ES
dc.titleA noninvasive method for time-lapse imaging of microbial interactions and colony dynamicses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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