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Enhancing shoot recovery from transgenic avocado somatic embryos
dc.contributor.author | Palomo-Ríos, Elena | |
dc.contributor.author | Vidoy-Mercado, Isabel | |
dc.contributor.author | Barceló-Muñoz, Araceli | |
dc.contributor.author | Mercado, Jose Angel | |
dc.contributor.author | Pliego-Alfaro, Fernando | |
dc.date.accessioned | 2013-09-04T06:20:51Z | |
dc.date.available | 2013-09-04T06:20:51Z | |
dc.date.issued | 2013-06-02 | |
dc.identifier.citation | 8th International Symposium on In vitro culture and Horticultural Breeding, University of Coimbra, Portugal, June 2-7, Libro de Abstracts, pág.53 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10630/5675 | |
dc.description.abstract | Use of biotechnological tools in avocado (Persea americana Mill.) is hampered by difficulties in obtaining mature somatic embryos with an acceptable germination capacity. Use of semi-permeable cellulose acetate membranes on top of maturation medium has improved the quality of obtained embryos and their germination rate; however, in the case of transgenic embryos the conversion rate is still rather low. In this investigation, a protocol for recovery of transgenic plants has been developed. Mature avocado somatic embryos, over cellulose acetate membranes, were obtained and induced to germinate following the protocol described in Palomo-Ríos (2012). Some transgenic embryos developed buds ≤ 2 mm, which failed to elongate. For shoot recovery, cotyledons were partially removed and the embryonic axis cultured over 4 weeks in MS medium supplemented with either 1 mg/l BA, 1 mg/l TDZ or 1 mg/l BA and 1 mg/l TDZ. Highest shoot recovery was obtained in medium supplemented with 1 mg/l BA and 1 mg/l TDZ, 53.6±6.7% versus 23.2±5,7% and 39.6±6.4% in media supplemented with 1 mg/l BA or 1 mg/l TDZ, respectively. Afterwards, sprouted embryos were cultured over 4 additional weeks in MS medium supplemented with 1 mg/l BA. In some cases, resulting shoots could be induced to proliferate in GD medium (Gamborg et al., 1968) supplemented with 0.3 mg/l BA, while in other cases, they had to be recovered through micrografting onto in vitro germinated seedlings as described in Pliego-Alfaro and Murashige (1987). MS medium supplemented with 1mg/l BA and solidified with 6 g/l Sigma A-1296 agar, was used for micrografts. Shoots derived from micrografts were either induced to proliferate in GD medium with 0.3 mg/l BA or further grafted and cultured in liquid MS medium supplemented with 0.1 mg/l BA, using perlite as substrate. After 8-12 weeks, micrografts could be transferred to ex vitro conditions. References: Gamborg O.L., Muller, R.A., Iojima, K., (1968). Nutrient requirements of suspension cultures of soybean roots cells. Exp. Cell. Res. 50:151-158. Palomo-Ríos, E., (2012). Thesis: Regeneración y Transformación Genética de Aguacate (Persea americana Mill.). Ph.D. Dissertation, University of Málaga, Málaga Pliego-Alfaro, F., Murashige, T., (1987). Possible rejuvenation of adult avocado by graftage onto juvenile root stocks in vitro. Hort Sci. 22:1321-1324. | es_ES |
dc.description.sponsorship | AGL2011-30354-C02-01 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | International Society for Horticultural Science | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Plantas transgénicas | es_ES |
dc.subject.other | somatic embryogenesis, Persea americana , transgenic plants , micrografting | es_ES |
dc.title | Enhancing shoot recovery from transgenic avocado somatic embryos | es_ES |
dc.type | info:eu-repo/semantics/conferenceObject | es_ES |
dc.centro | Facultad de Ciencias | es_ES |