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dc.contributor.authorNarváez-Peláez, Manuel 
dc.contributor.authorMillón-Peñuela, Carmelo 
dc.contributor.authorFlores-Burgess, Antonio 
dc.contributor.authorSantín-Núñez, Luis Javier 
dc.contributor.authorParrado-Romero, Concepción 
dc.contributor.authorPuigcerver-Martínez, Araceli 
dc.contributor.authorBorroto Escuela, Dasiel Óscar
dc.contributor.authorFuxe, Kjell
dc.contributor.authorDíaz-Cabiale, Zaida 
dc.contributor.authorNarváez-Bueno, José Ángel 
dc.date.accessioned2013-10-02T06:58:16Z
dc.date.available2013-10-02T06:58:16Z
dc.date.issued2013-10-02
dc.identifier.urihttp://hdl.handle.net/10630/6010
dc.description.abstractWe have shown Galanin(GAL)/Neuropeptide Y Y1 receptor(Y1) interactions in the nucleus tractus solitarius and the arcuate nucleus. Since both peptides play an important role in mood disorders, the aim of this work was to study GAL/Y1 interactions in the amygdala(AMY), key nucleus for fear, mood, and motivation. We have combined the analysis of the expression of c-Fos immunoreactivity(c-Fos IR) with an autoradiographic study in the AMY. Groups of anaesthetized rats (n=4) received intracerebroventricular injections(icv) of GAL(3nmol) and the Y1 agonist Leu31-Pro34NPY(3nmol) alone or in combination, and were sacrificed 90 minutes after the injections. Immunohistochemical detection of c-Fos protein(1:5000) in AMY sections and stereological analysis were performed in: Basal(BA), lateral(LA), Central [lateral capsular subdivision(CeC), lateral intermediate subdivision(CeI), medial subdivision(CeM)] and the medial paracapsular intercalated(ITC) subnuclei of the AMY. For Autoradiography, rats (n=6) were sacrificed 15 minutes after icv injections of GAL(3nmol) and AMY sections were incubated with Y1 agonist [125I]-Leu31-Pro34-PPY (25 pM). Autoradiograms were analyzed using the NIH image analysis system. Student’s unpaired t- test and ANOVA followed by Newman-Keuls were used, respectively. We observed within the AMY that GAL increased c-Fos IR in ITC and CeC; the Y1 agonist induced both, an increase of c-Fos IR in BA and CeC and a decrease of c-Fos IR in LA and ITC. The coadministration of both peptides induced a specific effect in the ITC, significantly decreasing the c-Fos expression (P<0,05) induced by GAL or the Y1 agonist alone. Moreover, we observed that GAL significantly increased (p<0,05) the Y1 receptor agonist binding [I125]Leu31Pro34-PYY in the AMY. These results demonstrate an interaction between GAL and Y1 at the cellular and receptor level in the AMY and suggest that endogenous GAL and NPY system might interact to regulate emotional behaviours.es_ES
dc.description.sponsorshipSpanish CVI6476, TV3-Marató 090130/31/32 and Universidad de Málaga (Campus de Excelencia Internacional Andalucía Tech)es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectNúcleo amigdalinoes_ES
dc.subject.otherGalanines_ES
dc.subject.otherNPYes_ES
dc.subject.otherAmygdalaes_ES
dc.titleImmunohistochemical C-FOS expression and autoradiography to study galnin/neuropeptide y Y1 receptor-receptor interactions in the amygdalaes_ES
dc.typeinfo:eu-repo/semantics/otheres_ES
dc.centroFacultad de Medicinaes_ES


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