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dc.contributor.authorLópez-Jimena, Benjamín
dc.contributor.authorGarcía-Rosado, Esther 
dc.contributor.authorSouto-Pereira, Sandra
dc.contributor.authorInfante, Carlos
dc.contributor.authorBorrego-Garcia, Juan Jose 
dc.contributor.authorAlonso-Sanchez, Maria del Carmen 
dc.date.accessioned2013-12-05T07:36:37Z
dc.date.available2013-12-05T07:36:37Z
dc.date.issued2013-09-02
dc.identifier.urihttp://hdl.handle.net/10630/6741
dc.description.abstractIn this study, the susceptibility of 5-g juvenile European sea bass was evaluated by intramuscular injection (105 TCID50/g) using isolates belonging to the RGNNV and SJNNV genotypes as well as a reassortant isolate (RGNNV RNA1/SJNNV RNA2) obtained from Senegalese sole (Solea senegalensis). In these experimental infections, the cumulative mortality was determined. Furthermore, quantification of viral genome, by absolute real time PCR, and infective viral particles, by virus titration, was performed from brains of dead and survivor fish (30 days post-inoculation). In addition, anti-VNNV antibodies in sera from survivor animals were determined by indirect ELISA. Typical symptoms of VNN and mortality were only recorded in fish inoculated with the RGNNV (47% cumulative mortality) and the reassortant (33%) isolates. However, high levels of viral genome and infective viral particles were recorded in brain of survivor fish inoculated with the SJNNV isolate, although did not cause mortality or clinical signs. Specific antibody response, measured by indirect ELISA, was only observed in the VNNV-inoculated groups, with titres of 1/1024, 1/4096 and 1/8192 for RGNNV, SJNNV and reassortant inoculated animals, respectively.es_ES
dc.description.sponsorshipUNIVERSIDAD DE MÁLAGA. CAMPUS DE EXCELENCIA INTERNACIONAL ANDALUCÍA TECHes_ES
dc.language.isoenges_ES
dc.publisherEAFPes_ES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectVirología - Investigaciónes_ES
dc.titleSusceptibility of juvenile european sea bass (dicentrarchus labrax) to different viral nervousnecrosis virus (VNNV) isolateses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle16th EAFP international conference on diseases of fish and shellfishes_ES
dc.relation.eventplaceTampere, Finlandiaes_ES
dc.relation.eventdateSeptiembre 2013es_ES


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