JavaScript is disabled for your browser. Some features of this site may not work without it.

    Listar

    Todo RIUMAComunidades & ColeccionesPor fecha de publicaciónAutoresTítulosMateriasTipo de publicaciónCentrosEsta colecciónPor fecha de publicaciónAutoresTítulosMateriasTipo de publicaciónCentros

    Mi cuenta

    AccederRegistro

    Estadísticas

    Ver Estadísticas de uso

    DE INTERÉS

    Datos de investigaciónReglamento de ciencia abierta de la UMAPolítica de RIUMAPolitica de datos de investigación en RIUMASHERPA/RoMEODulcinea
    Preguntas frecuentesManual de usoDerechos de autorContacto/Sugerencias
    Ver ítem 
    •   RIUMA Principal
    • Investigación
    • Fisiología Humana, Histología Humana, Anatomía Patológica y Educación Física y Deportiva - (FHEFD)
    • FHEFD - Contribuciones a congresos científicos
    • Ver ítem
    •   RIUMA Principal
    • Investigación
    • Fisiología Humana, Histología Humana, Anatomía Patológica y Educación Física y Deportiva - (FHEFD)
    • FHEFD - Contribuciones a congresos científicos
    • Ver ítem

    Two Main Subtypes of Aldosterone-Producing Adrenocortical Adenomas by Morphological and Expression Phenotype

    • Autor
      Blanes, Alfredo; Al-Hashimi, Fatima; Diaz-Cano, Salvador J.
    • Fecha
      2014-04-25
    • Palabras clave
      Cáncer - Aspectos endocrinos
    • Resumen
      Background: Aldosteronism is still a considerable diagnostic challenge generally diagnosed in a 3-tiered system (initial screening, a confirmation of the diagnosis, and a determination of the specific subtype). Since the recognition that ¾ of cases are due to bilateral hyperplasia, the spectrum of adenomas needs further characterization to determine the origin of aldosterone secretion. Design: We selected unilateral aldosterone-producing adrenocortical adenomas (AP-ACA, 33) responsible of primary aldosteronism defined by WHO criteria from a consecutive series of 98 ACA. We analyzed the histological features (growth pattern, nuclear characteristics, cytoplasmic staining qualities) of the tumor and the expression profile by quantitative RT-PCR of key molecular players of glomerulosa differentiation (SFRP2, β-catenin, AT1R, CYP21 CYP11B2, NURR1 and NUR77) in both the tumor and the surrounding parenchyma. RNA was extracted, cleaned from normal and neoplastic tissues (RNeasy columns), first-strand cDNA synthesized using T7-(dT24)-oligomer and used as template for cRNA synthesis.. The peritumoral parenchyma was also evaluated for the cytohistological features of the glomurulosa and its extension into deep cortex/medulla and periadrenal soft tissues. Quantitative results were cross-validated (expression factor>2, significance<0.01). Variables were studied regarding morphological appearances of the tumor and the status of the peritumoral glomerulosa. Results: Two main groups of AP-ACA were identified morphologically with a corresponding molecular profile. AP-ACA composed predominantly of clear foamy cells (10) that revealed minimal expression of AT1R, CYP21 and CYP11B2 and AP-ACA composed predominantly of eosinophilic cells (23) expressing significantly high AT1R, CYP21 and CYP11B2. The peritumoral parenchyma revealed functional hyperplastic glomerulosa in 31 cases, more prominent and with extra-adrenal extension in clear cell AP-ACA. Conclusions: The common presence of peritumoral hyperplasia suggests a proliferative response of cells to unidentified paracrine/autocrine factor as main mechanism in AP-ACA, which are not involved in glomerulosa differentiation in the clear cell subtype. Clear cell AP-ACA causes a syndrome of aldosteronism characterized by histologic features intermediate between adrenal adenoma and adrenal hyperplasia. Category: Endocrine Pathology
    • URI
      http://hdl.handle.net/10630/7435
    • Compartir
      RefworksMendeley
    Mostrar el registro completo del ítem
    Ficheros
    Two Main Subtypes of Aldosterone.pdf (102.3Kb)
    Colecciones
    • FHEFD - Contribuciones a congresos científicos

    Estadísticas

    Ver Estadísticas de uso
    Academic Search
    Buscar en Dimension
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
     

     

    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA