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dc.contributor.authorBlanes, Alfredo
dc.contributor.authorAl-Hashimi, Fatima
dc.contributor.authorDiaz-Cano, Salvador J.
dc.date.accessioned2014-04-25T12:11:57Z
dc.date.available2014-04-25T12:11:57Z
dc.date.created2014-04-24
dc.date.issued2014-04-25
dc.identifier.urihttp://hdl.handle.net/10630/7435
dc.description.abstractBackground: Aldosteronism is still a considerable diagnostic challenge generally diagnosed in a 3-tiered system (initial screening, a confirmation of the diagnosis, and a determination of the specific subtype). Since the recognition that ¾ of cases are due to bilateral hyperplasia, the spectrum of adenomas needs further characterization to determine the origin of aldosterone secretion. Design: We selected unilateral aldosterone-producing adrenocortical adenomas (AP-ACA, 33) responsible of primary aldosteronism defined by WHO criteria from a consecutive series of 98 ACA. We analyzed the histological features (growth pattern, nuclear characteristics, cytoplasmic staining qualities) of the tumor and the expression profile by quantitative RT-PCR of key molecular players of glomerulosa differentiation (SFRP2, β-catenin, AT1R, CYP21 CYP11B2, NURR1 and NUR77) in both the tumor and the surrounding parenchyma. RNA was extracted, cleaned from normal and neoplastic tissues (RNeasy columns), first-strand cDNA synthesized using T7-(dT24)-oligomer and used as template for cRNA synthesis.. The peritumoral parenchyma was also evaluated for the cytohistological features of the glomurulosa and its extension into deep cortex/medulla and periadrenal soft tissues. Quantitative results were cross-validated (expression factor>2, significance<0.01). Variables were studied regarding morphological appearances of the tumor and the status of the peritumoral glomerulosa. Results: Two main groups of AP-ACA were identified morphologically with a corresponding molecular profile. AP-ACA composed predominantly of clear foamy cells (10) that revealed minimal expression of AT1R, CYP21 and CYP11B2 and AP-ACA composed predominantly of eosinophilic cells (23) expressing significantly high AT1R, CYP21 and CYP11B2. The peritumoral parenchyma revealed functional hyperplastic glomerulosa in 31 cases, more prominent and with extra-adrenal extension in clear cell AP-ACA. Conclusions: The common presence of peritumoral hyperplasia suggests a proliferative response of cells to unidentified paracrine/autocrine factor as main mechanism in AP-ACA, which are not involved in glomerulosa differentiation in the clear cell subtype. Clear cell AP-ACA causes a syndrome of aldosteronism characterized by histologic features intermediate between adrenal adenoma and adrenal hyperplasia. Category: Endocrine Pathologyes_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCáncer - Aspectos endocrinoses_ES
dc.titleTwo Main Subtypes of Aldosterone-Producing Adrenocortical Adenomas by Morphological and Expression Phenotypees_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Medicinaes_ES
dc.relation.eventtitle103RD USCAPes_ES
dc.relation.eventplaceSAN DIEGO CALIFORNIAes_ES
dc.relation.eventdate1-7 DE MARZO 2014es_ES


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