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dc.contributor.authorSanchez-Jimenez, Francisca Maria 
dc.date.accessioned2014-05-23T08:41:46Z
dc.date.available2014-05-23T08:41:46Z
dc.date.created2014-05-07
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10630/7538
dc.description.abstractHistamine (Hia) is the most multifunctional biogenic amine. It is synthetized by histidine decarboxylase (HDC) in a reduced set of mammalian cell types. Mast cells and histaminergic neurons store Hia in specialized organelles until the amine is extruded by exocytosis; however, other immune and cancer cells are able to produce but not store Hia. The intracellular effects of Hia are still not well characterized, in spite of the physiopathological relevance of the amine. Multiple functional relationships exist among Hia metabolism/signaling elements and those of other biogenic amines, including growth-related polyamines. In a previous work, we obtained the first insights for an inhibitory effect of newly synthetized Hia on the cell cycle of non-fully differentiated mammalian cells. In the present work, we describe progress on this line. HEK293 cells were transfected to express active and inactive versions of GFP-human HDC. Cells expressing GFP fusion proteins were sorted by flow cytometry and their relative levels of protein expression related to cell signaling were measured using an Antibody Microarray. Experimental results were analyzed in terms of protein-protein and functional interaction networks. Key facts were experimentally validated by different approaches. The analyses uncover cross-talk mechanisms among biogenic amine-related pathways and provide new clues on the molecular mechanisms underlying the regulatory effects of intracellular newly synthetized Hia on cell proliferation/survival, cell trafficking and protein turnover. This information is especially interesting for emergent and orphan immune and neuroinflammatory diseases.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Granted by CIBERER (ISCIII, Spain), SAF2011-26518 (MINECO, Spain) and CVI-6585 (Junta de Andalucía).es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHistaminaes_ES
dc.titleNew findings on the cellular effects of newly synthetized histamine in mammalian cellses_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleEHRS 43th Annual Meetinges_ES
dc.relation.eventplaceLyon, Franciaes_ES
dc.relation.eventdate7-10 Mayo 2014es_ES


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