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dc.contributor.authorNarváez, Manuel 
dc.contributor.authorMillón Peñuela, Carmelo
dc.contributor.authorBorroto-Escuela, Dasiel
dc.contributor.authorFlores-Burgess, Antonio
dc.contributor.authorSantín Núñez, Luis
dc.contributor.authorParrado, Concepción
dc.contributor.authorGago, Belén
dc.contributor.authorPuigcerver, Araceli
dc.contributor.authorFuxe, Kjell
dc.contributor.authorNarváez, José Ángel 
dc.contributor.authorDíaz-Cabiale, Zaida 
dc.date.accessioned2014-07-10T11:26:14Z
dc.date.available2014-07-10T11:26:14Z
dc.date.created2014-07-10
dc.date.issued2014-07-10
dc.identifier.urihttp://hdl.handle.net/10630/7801
dc.description.abstractWe have shown that Galanin(GAL) interacts with Neuropeptide Y Y1 receptor(Y1) at behavioural, cellular and receptor levels in the Amygdala. Since both peptides play an important role in mood disorders, the aim of this work was to study the GALR2/Y1 heterodimer formation within the amygdala(AMY), key nucleus for fear, mood, and motivation. We performed in situ proximity ligation assay(PLA) in the Amygdala and also in HEK-293 cells. Amygdala sections were incubated with anti-GALR2 Rabbit(1:100) and anti- NPYY1R Goat(1:200). In transiently transfected HEK293T cells coexpressing Y1R(3xHA) and GALR2 mouse anti-HA(1:5000) and rabbit anti-GALR2(1:500) were used. In both cases PLA signals were detected with PLA PLUS or MINUS probes for rabbit or goat/mouse antibodies. PLA signals were visualized by using a confocal microscope Leica TCS-SL confocal microscope(Leica). PLA-positive red clusters were found in large number of cells of the Basolateral, Intercalated and Central nuclei of the Amygdala. No PLA clusters were observed in lateral corpus callosum, an area that seems to lack of GALR2 receptor. In HEK293T cells we reproduced the results obtained in the Amygdala, showing PLA-positive red clusters in large number of cells. The fact that we found PLA-positive red clusters in a large number of cells not only in the Amygdala but also in the HEK293T cells line give indications that GALR2 and NPYY1R are forming GALR2/NPYY1R heterodimers, providing a novel mechanism with implications in behavior, particularly in the anxiety response.es_ES
dc.description.sponsorshipUniversidad de Málaga, Campus de Excelencia Internacional Andalucía Tech. Spanish CVI6476es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectNúcleo amigdalinoes_ES
dc.subjectNeuropéptidoses_ES
dc.subject.otherGalanines_ES
dc.subject.otherNeuropeptide Yes_ES
dc.subject.otherAmygdalaes_ES
dc.subject.otherAnxietyes_ES
dc.titleNPYY1 /Galanin 2 heterodimers in the amygdala: a proximity ligation assay studyes_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Medicinaes_ES
dc.relation.eventtitle9th FENS Forum of Neuroscience - Milan 2014es_ES
dc.relation.eventplaceMilan, Italiaes_ES
dc.relation.eventdate05/07/2014es_ES


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