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    • Microbiología - (MIC)
    • MIC - Contribuciones a congresos científicos
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    •   RIUMA Principal
    • Investigación
    • Microbiología - (MIC)
    • MIC - Contribuciones a congresos científicos
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    Deciphering molecular mechanisms underlaying biological control of the fungal pathogen Rosellinia necatrix by Pseudomonas pseudoalcaligenes AVO110

    • Autor
      Cazorla-López, Francisco ManuelAutoridad Universidad de Málaga; Pérez-Martínez, Isabel; Crespo-Gómez, José IgnacioAutoridad Universidad de Málaga; Pintado-Calvillo, Adrian; Pliego-Prieto, Clara; De-Vicente-Moreno, AntonioAutoridad Universidad de Málaga; Ramos-Rodríguez, Cayo JuanAutoridad Universidad de Málaga
    • Fecha
      2014-07
    • Palabras clave
      Plagas agrícolas - Control biológico; Pseudomonas
    • Resumen
      Pseudomonas pseudoalcaligenes AVO110 was selected as an efficient avocado root tip colonizer, displaying antagonism towards Rosellinia necatrix, the causal agent of avocado white root rot. The most likely biocontrol mechanisms used by this strain is competition for niches and nutrients. In fact, AVO110 is able to colonise competitively the surface of fungal hyphae, using fungal exudates. In a previous study, we used signature-tagged mutagenesis (STM) to screen a library of AVO110 transposon mutants for their ability to grow in Rosellinia exudates (BM-RE medium). A pool of 26 growth-attenuated mutants (GAM) was identified. In this work, we used the recently obtained draft genome sequence of AVO110 to analyse the genetic context of the genes affected by the transposon in seven GAM mutants. Among the traits affected, we have identified homologs of an exodeoxyribonuclease, a GGDEF/EAL/PAS protein, a transcriptional regulator, a histidine kinase, a putative enterotoxin, a peptidase and a putative small RNA. Phenotypic characteristics such as biofilm formation, persistence and colonisation of avocado roots and colonisation of fungal hyphae were studied. Mutants affected in the transcriptional regulator and the GGDEF/EAL/PAS proteins were altered in all features analysed when compared to the wild type strain. The remaining mutants presented variations in persistence and colonisation of fungal and root surfaces, except for that affected in a putative enterotoxin, which was only altered in fungal colonisation. Finally, quantitative RT-PCR experiments were performed in BM-RE to evaluate the expression dynamics of these genes in the presence of the fungal metabolites along the time. Results showed no evident correlation between gene expression and phenotypic behaviour.
    • URI
      http://hdl.handle.net/10630/7854
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    Ficheros
    P133.pdf (65.49Kb)
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    • MIC - Contribuciones a congresos científicos

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    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
     

     

    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA
    REPOSITORIO INSTITUCIONAL UNIVERSIDAD DE MÁLAGA