Pseudomonas pseudoalcaligenes AVO110 was selected as an efficient avocado root tip
colonizer, displaying antagonism towards Rosellinia necatrix, the causal agent of avocado white
root rot. The most likely biocontrol mechanisms used by this strain is competition for niches and
nutrients. In fact, AVO110 is able to colonise competitively the surface of fungal hyphae, using
fungal exudates. In a previous study, we used signature-tagged mutagenesis (STM) to screen a
library of AVO110 transposon mutants for their ability to grow in Rosellinia exudates (BM-RE
medium). A pool of 26 growth-attenuated mutants (GAM) was identified.
In this work, we used the recently obtained draft genome sequence of AVO110 to analyse the
genetic context of the genes affected by the transposon in seven GAM mutants. Among the
traits affected, we have identified homologs of an exodeoxyribonuclease, a GGDEF/EAL/PAS
protein, a transcriptional regulator, a histidine kinase, a putative enterotoxin, a peptidase and a
putative small RNA. Phenotypic characteristics such as biofilm formation, persistence and
colonisation of avocado roots and colonisation of fungal hyphae were studied. Mutants affected
in the transcriptional regulator and the GGDEF/EAL/PAS proteins were altered in all features
analysed when compared to the wild type strain. The remaining mutants presented variations in
persistence and colonisation of fungal and root surfaces, except for that affected in a putative
enterotoxin, which was only altered in fungal colonisation.
Finally, quantitative RT-PCR experiments were performed in BM-RE to evaluate the expression
dynamics of these genes in the presence of the fungal metabolites along the time. Results
showed no evident correlation between gene expression and phenotypic behaviour.
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