Mostrar el registro sencillo del ítem
Preparation, photophysical properties and cell image of Naphthalimide-based fluorescent nanostructures for biomedical applications: BAPAD dendrimeric antigens and silica nanoparticles
dc.contributor.author | Mesa, Pablo | |
dc.contributor.author | Collado-Martín, Daniel | |
dc.contributor.author | Vida-Pol, Yolanda | |
dc.contributor.author | Nájera-Albendín, Francisco | |
dc.contributor.author | Pérez-de-Inestrosa-Villatoro, Ezequiel | |
dc.date.accessioned | 2014-07-17T10:04:53Z | |
dc.date.available | 2014-07-17T10:04:53Z | |
dc.date.created | 2014 | |
dc.date.issued | 2014-07-17 | |
dc.identifier.uri | http://hdl.handle.net/10630/7857 | |
dc.description.abstract | We present here the synthesis and characterization of BAPAD [1] fluorescent Dendrimeric-Antigens (DeAn), to be applied in basophil activation as a test to detect drug allergy reactions. A second generation BAPAD dendrimer using cystamine as core that can be reducced to obtain two dendrons with a thiol group in the focal point, useful to attach a fluorescent probe, have been synthesized. The free amine groups on the surface of the dendrimer were functionalized with an amoxiciloyl group (AXO), the allergenic determinant to the beta-lactam antibiotic amoxicillin. The fluorescent DeAn (F-DeAn) has been fully characterized by NMR and MS techniques, and their fluorescent properties well established in aqueous biological media. F-DeAn without the haptenic moieties at the periphery has been also obtained and fully characterized as a control assay. Both molecules have been also characterized using molecular dynamics simulation calculations. We show here how these dendrimeric structures interacts with dendritic cells and are internalized by them. In a secon approximation, Naphthalimide-fluorscent silica nanoparticles have been prepared and characterized, to be used also in basophil activation test. | es_ES |
dc.description.sponsorship | Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Fotoquímica | es_ES |
dc.subject.other | Photochemistry | es_ES |
dc.title | Preparation, photophysical properties and cell image of Naphthalimide-based fluorescent nanostructures for biomedical applications: BAPAD dendrimeric antigens and silica nanoparticles | es_ES |
dc.type | info:eu-repo/semantics/conferenceObject | es_ES |
dc.centro | Facultad de Ciencias | es_ES |
dc.relation.eventtitle | 4as Jornadas Ibéricas de Fotoquímica | es_ES |
dc.relation.eventplace | Lisboa, Portugal | es_ES |
dc.relation.eventdate | 29/06-02/04/2014 | es_ES |