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dc.contributor.authorGrondona, Jesús M
dc.contributor.authorGranados-Durán, Pablo
dc.contributor.authorNavarro Dueñas, Isabel
dc.contributor.authorFernández-Llebrez, Pedro 
dc.contributor.authorLópez-Ávalos, María Dolores 
dc.date.accessioned2014-07-21T11:16:53Z
dc.date.available2014-07-21T11:16:53Z
dc.date.created2014-07
dc.date.issued2014-07-21
dc.identifier.urihttp://hdl.handle.net/10630/7875
dc.description.abstractMulticiliated ependymal cells form an epithelium lining most of the ventricular cavities of vertebrates brain. Although considered postmitotic and completely differentiated, ependymal cells maintain some phenotypic characteristics of neural stem cells. Thereby, under specific conditions they behave as neural stem cells, developing radial glia characteristics, and undergoing asymmetric division. Our group is searching for factors that promote dedifferentiation of ependymal cells in vitro. We developed a simple method to obtain pure cultures of non-adherent multiciliated ependymal cells from adult rats. These cultures were used to investigate the effect of FGF2 on the differentiation state and the aggregation of ependymal cells. Thus, FGF2 treated ependymal cells lose cilia and hence mobility, and after 7 days they aggregate to form irregular spheres (diameter ≥ 20-30 μm). Such changes were not observed when EFG was used instead of FGF2. To assess the specificity of FGF2 action on cell aggregation, the FGF receptor inhibitor PD166866 and an anti-FGF2 neutralizing antibody were used. In both conditions the aggregative effect of FGF2 was abolished. No cell proliferation was observed during sphere formation, at least in such experimental conditions. Spheres were analyzed by immunocytochemistry using radial glia markers. They were positive for GFAP, vimentin, BLBP and GLAST. These data suggest that FGF2 promotes the identity loss in multiciliated ependymal cells in vitro, which are transformed into cells with radial glia features.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectNeurogliaes_ES
dc.subjectEpéndimoes_ES
dc.subject.otherEpendymales_ES
dc.subject.otherEpendymaes_ES
dc.subject.otherFGF2es_ES
dc.subject.otherRadial glial cellses_ES
dc.titleFGF2, but not EGF, Induces multiciliated ependymal cells to dedifferentiate and adopt radial glial features in vitroes_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitle9th FENS Forum of Neurosciencees_ES
dc.relation.eventplaceMilánes_ES
dc.relation.eventdate5-9 july 2014es_ES


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