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dc.contributor.authorVela-Corcía, David
dc.contributor.authorBautista, Rocío
dc.contributor.authorDe Vicente, Antonio
dc.contributor.authorClaros-Diaz, Manuel Gonzalo 
dc.contributor.authorSpanu, Pietro
dc.contributor.authorPérez-García, Alejandro
dc.date.accessioned2014-07-24T10:03:58Z
dc.date.available2014-07-24T10:03:58Z
dc.date.created2014-07
dc.date.issued2014-07-24
dc.identifier.urihttp://hdl.handle.net/10630/7902
dc.descriptionComunicación presentada en formato panel en la sesión "Large-scale (omics) approaches"es_ES
dc.description.abstractThe cucurbit powdery mildew fungus Podosphaera fusca, is a major limiting factor for cucurbit production worldwide. Despite its agronomic and economic importance, very little is known about fundamental aspects of P. fusca biology such as obligate biotrophy and pathogenesis. In order to design novel and more durable control strategies, genomic information of P. fusca is needed. In this work we aimed to analyse the epiphytic transcriptome of P. fusca as starting point. Total RNA was isolated from mycelia and conidia, and the corresponding cDNA library was sequenced using a 454 GS FLX platform. Annotation data was acquired for 62.6% of the assembled sequences, identifying 9,713 putative genes with different orthologues. In the transcript data set, the most represented protein functions were those involved in gene expression, protein metabolism, regulation of biological process and organelle organization. Our analysis also confirmed the existence of “missing ascomycete core genes” (MACGs) found in other powdery mildew species. After analysis of the pool of fungal secreted proteins, 118 putative secreted proteins were identified, including 35 “candidate secreted effector proteins” (CSEPs) specific for P. fusca. In order to validate the in silico assembly, the expression profile of some CSEPs was analysed, which was consequent with a canonical effector expression pattern, with a maximum of expression at the beginning of the infection process 24-48 h after inoculation. Our data open the genomics era of this very important cucurbit pathogen.es_ES
dc.description.sponsorshipUniversidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Plan Nacional Plan I+D+I del antiguo Ministerio de Ciencia e Innovación (AGL2010-21848-CO2-01), cofinanciado con fondos FEDER (UE).es_ES
dc.language.isoenges_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectMildiues_ES
dc.subjectCucurbitáceases_ES
dc.subject.otherSecretomees_ES
dc.subject.otherAndidate effectorses_ES
dc.subject.otherPowdery mildewses_ES
dc.subject.otherCucurbitses_ES
dc.titleThe epiphytic transcriptome of Podosphaera fusca and its predicted secretomees_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.centroFacultad de Cienciases_ES
dc.relation.eventtitleXVI International Congress on Molecular Plant-Microbe Interactionses_ES
dc.relation.eventplaceRodhes, Greecees_ES
dc.relation.eventdateJuly 2014es_ES


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