Quantitative real-time polymerase chain reaction improves conventional microbiological diagnosis in an outbreak of brucellosis due to ingestion of unpasteurized goat cheese.
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Abstract
Rapid diagnosis of individuals involved in brucellosis outbreaks can sometimes be difficult with conventional microbiological techniques. We analyzed, for the first time, the diagnostic yield of a real-time polymerase chain reaction (PCR) assay in a family outbreak of brucellosis due to consumption of unpasteurized goat cheese. PCR correctly identified all symptomatic cases.
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Colmenero, J. D., Clavijo, E., Morata, P., Bravo, M. J., & Queipo-Ortuño, M. I. (2011). Quantitative real-time polymerase chain reaction improves conventional microbiological diagnosis in an outbreak of brucellosis due to ingestion of unpasteurized goat cheese. Diagnostic Microbiology and Infectious Disease, 71(3), 294–296. https://doi.org/10.1016/j.diagmicrobio.2011.06.016